IL-2基因直接转移促进CD4^+缺损小鼠免疫功能的恢复  

Recovery of the immune function in CD4 + deficient mice by IL 2 gene transfer

作　　者：刘炜[1] 殷金珠[1] 

机构地区：[1]北京医科大学免疫系

出　　处：《中华微生物学和免疫学杂志》1998年第3期239-242,共4页Chinese Journal of Microbiology and Immunology

基　　金：美国洛氏中华医学基金会资助

摘　　要：目的探讨ＩＬ－２基因直接转移治疗小鼠ＣＤ４缺陷的可能性。方法ｐＣＩ－ｈＩＬ－２质粒ＤＮＡ直接注入ＣＤ４缺陷小鼠大腿肌肉内。用免疫组化法检测ｈＩＬ－２蛋白的表达，同时测定小鼠的免疫功能。结果ｐＣＩ－ＩＬ－２质粒ＤＮＡ可在注射部位肌纤维内表达ＩＬ－２蛋白。ＣＤ４缺陷小鼠脾细胞中ＣＤ４＋Ｔ细胞增多，脾细胞对有丝分裂素ＣｏｎＡ增殖反应明显增强。对ｓＲＢＣ的免疫应答无论是体液免疫或细胞免疫应答均高于对照组（Ｐ＜０．０１）。结论ｐＣＩ－ＩＬ－２质粒ＤＮＡ直接肌内注射可部分纠正ＣＤ４缺陷小鼠的免疫功能。Objective To explore the possibility of correcting the immunodeficiency of CD4 deficient mice through IL 2 gene direct transfer. Method pCI hIL 2 plasmid DNA was injected into muscle of CD4 deficient mice.The mice injected with pCI IL 2 plasmid DNA were examined immune function and expression of IL 2 protein.Result (1)The result of immunohistochemistry suggested that myofiber had expressed the hIL 2 protein.(2) Injection of pCI IL 2 plasmid DNA result in an increased number of CD4 + T cell and enhanced the proliferation response of splenocytes to Con A.(3)The immune response of mice injected with pCI hIL 2 plasmid DNA to sRBC are much higher than the control mice. Conclusion All the results indicated that the expressed hIL 2 in muscle cell after direct DNA injection could partly correct the immunodeficiency of CD4 deficient mice.

关 键 词：基因转移 CD4缺陷 白细胞介素-2 艾滋病 

分 类 号：R593.305[医药卫生—内科学] R512.910.5[医药卫生—临床医学]