rTNFα、rIL-1及rIL-1ra对人脐静脉内皮细胞释放IL-1的影响  

EFFECT OF IL 1 RELEASE OF HUVEC INDUCED BY rTNFα,rIL 1 AND rIL 1ra

作　　者：张庆殷[1,2] 侯桂华[1,2] 曹容华 孙汶生[1,2] 宋静 

机构地区：[1]山东医科大学附属医院检验科 [2]山东医科大学基础医学院免疫学教研室

出　　处：《山东医科大学学报》1998年第2期107-111,共5页Acta Academiae Medicinae Shandong

基　　金：山东省自然科学基金会青年科学基金

摘　　要：用体外原代培养的人脐静脉内皮细胞（ＨＵＶＥＣ）观察ｒＴＮＦα、ｒＩＬ－１对ＨＵＶＥＣ产生ＩＬ－１的诱导效应及重组ＩＬ－１受体拮抗剂（ｒＩＬ－１ｒａ）的抑制效应。结果表明，ｒＴＮＦα以剂量依赖的方式促进ＨＵＶＥＣ释放ＩＬ－１；刺激８ｈＩＬ－１的释放活性显著提高，２４ｈ达峰值。ｒＩＬ－１也可促进ＨＵＶＥＣ释放ＩＬ－１，随ｒＩＬ－１浓度的增加，ＩＬ－１的释放活性也增加，刺激４ｈ，ＩＬ－１的释放活性即可明显提高（Ｐ＜０．０１）。ｒＩＬ－１ｒａ可抑制由ｒＩＬ－１引起的ＩＬ－１释放，提高ｒＩＬ－１ｒａ的浓度，上清液中ＩＬ－１活性相应减低，当ｒＩＬ－１ｒａ浓度达１０ｍｇ／Ｌ时。The studies were made on the effect of production of IL 1 in HUVEC induced by rTNFα,rIL 1 and the inhibitory effect of rIL 1ra.The results showed that rTNFα promoted HUVEC to release IL 1 in a dose dependent manner.When HUVEC was stimulated,the secretion of IL 1 rose significantly 8 hours later and reached peak 24 hours later.Recombined IL 1(rIL 1)promoted HUVEC to produce IL 1.The production of IL 1 rose in proportion to rIL 1 dose dependently.The activity of release of IL 1 rose obviously 4 hours after HUVEC was stimulated( P <0.01).rIL 1ra inhibited IL 1 secretion of HUVEC induced by rIL 1.Activity of IL 1 in supernatant decreased when the concentration of rIL 1ra increased at the same time.When rIL 1ra reached 10mg/L.activity of IL 1 could be inhibited almost completely.

关 键 词：脐静脉 内皮细胞 白细胞介素1 受体 拮抗剂 

分 类 号：R329.12[医药卫生—人体解剖和组织胚胎学]