柴芩肾安方对IgA肾病大鼠肾组织TGF-β_1和Smad 7的影响  

Effects of Chaiqinshenan Recipe on TGF-β_1 and Smad 7 in Renal Tissues of IgA Nephropathy Rats

作　　者：彭文[1] 刘育军[1] 赵燕俐[1] 孙仲伦[1] 王浩[1] 王红婷 黄文政[2] 

机构地区：[1]上海中医药大学附属普陀医院肾内科,上海200062 [2]天津中医药大学第一附属医院肾内科,天津300193

出　　处：《中国中西医结合肾病杂志》2009年第1期18-20,I0002,共4页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:探讨柴芩肾安方对IgAN大鼠肾组织TGF-β1和Smad 7表达的影响。方法:采用灌服并定时静脉注射BSA复合感染SEB的方法制成大鼠IgAN模型,并用柴芩肾安方治疗,以氯沙坦为对照。第15周末,观察肾组织形态学变化和免疫复合物的沉积情况;采用免疫组化和RT-PCR的方法,分别检测肾组织TGF-β1和Smad 7蛋白及其mRNA的表达。结果:与正常组相比,模型组大鼠肾小球系膜细胞和系膜基质轻度增生,伴见弥漫的IgA和少量IgG沉积;TGF-β1和Smad 7蛋白及其mRNA表达均明显升高(P<0.01)。柴芩肾安方治疗后上述指标均明显减轻(P<0.01),且作用效果与氯沙坦类似。结论:抑制肾组织TGF-β1和Smad 7蛋白及其mRNA的表达,可能是柴芩肾安方延缓IgAN肾脏病变进展的机制之一。Objective：To investigate the effects of Chaiqinshenan Recipe （CQS） on protein and mRNA expressions of TGF - β1 and Smad7 in renal tissues of IgAN rats. Methods：Rat IgAN models were established by oral admininistration and time- controlled injection of BSA and SEB into phallic vein. The treated rats were given with CQS and rats treated with Losartan were set as the control. At the end of 15th week, renal morphological changes and deposits of immune complexes were observed. Immunohistochemistry and RT - PCR were used to examine the protein and rnRNA expressions of TGF - β1 and Smad 7 in renal tissues. Results. Compared to the normal group, the mesangial cells proliferated and mesangial matrix increased lightly with diffused IgA and little IgG deposition. Also the expression levels of protein and mRNA of TGF - β1 and Smad 7 in the model group were remarkably up - regulated （ P 〈 0.01 ）. CQS administration relieved all changes （ P 〈 0.01 ）, which resembled the same effects of Losartan. Conclusion： It is indicated that CQS can restrain the protein and mRNA expressions of TGF - β1 and Smad 7 to retard the development of renal pathologic changes in IgAN rats.

关 键 词：柴芩肾安方 IGA肾病 转化生长因子-Β1 SMAD 7 

分 类 号：R285.5[医药卫生—中药学]