鼻腔NK／T细胞淋巴瘤中p53和p21表达及其与细胞增殖和凋亡的关系  

Expressions of p53 and p21 in nasal NK/T-cell lymphoma and their relationship with the proliferation and apoptosis of cells

作　　者：徐刚[1] 王槐富[1] 何刚[1] 陈德忠[2] 

机构地区：[1]四川省医学科学院 四川省人民医院耳鼻咽喉科,成都610072 [2]四川省人民医院病理科

出　　处：《临床耳鼻咽喉头颈外科杂志》2009年第2期73-76,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：四川省卫生厅科研课题(No:080368)

摘　　要：目的:探讨p53、p21蛋白在鼻腔NK/T细胞淋巴瘤(nasal NK/T-cell lymphoma,NKTL)中的表达,及其与细胞增殖和凋亡的关系。方法:取62例NKTL组织构建组织芯片,应用免疫组织化学方法检测p53、p21、Ki67蛋白的表达情况,TUNEL法原位凋亡检测,计算增殖指数(proliferation index,PI)和凋亡指数(apoptosis in-dex,AI)。结果:①p53、p21蛋白在NKTL中表达的阳性率分别为79.03%、58.06%;②p53在Ann ArborⅠ、Ⅱ、Ⅲ、Ⅳ期患者中表达的阳性率分别为69.57%、75.00%、86.67%、100.00%,p21在Ann ArborⅠ、Ⅱ、Ⅲ、Ⅳ期患者中表达的阳性率分别为47.83%、56.25%、60.00%、87.50%,随着肿瘤进展,二者表达阳性率逐渐升高,差异有统计学意义(P<0.05);③p53在大、中、小型瘤细胞患者中表达的阳性率分别为92.86%、78.95%、53.33%,p21在大、中、小型瘤细胞患者中表达的阳性率分别为67.86%、57.89%、40.00%,随着肿瘤细胞增大,二者表达阳性率逐渐升高,差异有统计学意义(P<0.05);④p53和p21二者表达之间统计学上具有明显的相关性(P<0.05);随着p53、p21蛋白表达强度的增加,肿瘤临床分期增高,肿瘤细胞增大,PI亦逐渐升高,它们均与PI呈正相关(P<0.05),而与AI无关联性(P>0.05)。结论:p53、p21蛋白表达与NKTL的发生、发展密切相关,联合检测Ki67表达,可作为评估该病患者肿瘤细胞增殖、侵袭性等生物学行为的良好指标。Objective： To investigate the significance of expressions of p53 and p21 in nasal NK/T-cell lymphoma（NKTL） and their relationship with cell proliferation and apoptosis. Method：Sixty-two cases of NKTL were examined for p53, p21 and Ki67 proteins by means of tissue microarray technique, TUNEL and immunohistochemis try. The proliferation index （PI） was determined by expression of Ki67 proteins. Result： The positive expression rates of p53 and p21 proteins in NKTL were 79.03% and 58.06% respectively. The positive expression rates of p53 in Ann Arbor stage Ⅰ , Ⅱ ,Ⅲ and Ⅳ NKTL were 69.57% ,75%,86.67% and 100% respectively, while those of p21 were 47. 83% ,56.25% ,60% and 87.50%. With the progression of tumor, the positive expression rates of p53 and p21 proteins gradually increased. And there were significant differences between them（ P〈0.05）. The positive expression rates of p53 in NKTL with large, medium and small size tumor cells were 92.86%, 78.95% and 53.33% respectively, while those of p21 were 67.86%, 57.89% and 40.00%. With the expanding of tumor cells, the positive expression rates of p53 and p21 proteins gradually increased. And there were significant differences between them（ P〈0.05）. The expression of p53 was positively correlated with the expression of p21（ P〈0.05）. The intensity of p53 and p21 expression, the Ann Arbor stage and the size of tumor cell all were positively correlated with PI（Spearman correlation analysis, P〈0.05）, while no correlation with AI（ P 〈0.05）. Conclusion： The expressions of p53, p21 and Ki67 proteins are closely related with the pathogenesis and progression of NKTL. Combined detection of p53, p21 and Ki67 is a good marker to judge the biological behavior of NKTL, such as the proliferation and the invasiveness of the tumor.

关 键 词：非霍奇金淋巴瘤 NK/T细胞 增殖 细胞凋亡 P53蛋白 P21蛋白 

分 类 号：R765[医药卫生—耳鼻咽喉科]