地噻咪松诱导乳鼠胸腺细胞凋亡的研究  

[WT4”HZ〗THE STUDY ON DEX INDUCED APOPTOSIS OF IMMATURE MOUSE THYMOCYTES

作　　者：梁福佑[1] 陈哲生[1] 李金蕾[1] 赵俊萍[1] 

机构地区：[1]首都医科大学细胞生物学教研室

出　　处：《动物学杂志》1998年第3期3-5,共3页Chinese Journal of Zoology

摘　　要：本文用透射电镜的方法，研究了地噻咪松诱导乳鼠胸腺细胞的凋亡，并确立了乳鼠胸腺细胞中凋亡细胞形态学特征；含溴化乙锭的琼脂多糖（１５％）电泳检测乳鼠胸腺细胞中凋亡细胞的断裂ＤＮＡ；末端标记法（ＴＵＮＥＬ法）直接、持异的标记乳鼠胸腺细胞中凋亡细胞断裂ＤＮＡ。获得地噻咪松诱导乳鼠胸腺细胞中凋亡细胞，以及透射电镜下可见凋亡细胞的典型形态学特征；ＤＮＡ琼脂糖电泳呈典型梯状条带；ＴＵＮＥＬ法中凋亡细胞着染，褐色颗粒沉着。提示不同浓度的地噻咪松可诱导乳鼠胸腺细胞凋亡，稍加改进ＴＵＮＥＬ法后，显示结果比较好。Objetive · the aim of the study is to determine the effect of DEX induced apoptosis of immature mouse thymocytes.Methods · The apoptotic cells of morphologic characterization were established by the Transmission Electron Microscope.The purified DNA of immature mouse thymocytes was electrophoresed using a 1 5% agarose gel with ethidium bromide staining.The TUNEL method is based on direct,specific,labeling of DNA breaks in nuclei,in situ.Results · DEX induced apoptosis of immature mouse thymocytes,showed a typical character of morphology,laddering pattern of DNA,and TUNEL stained cell.Conclusion · We thought that the different concentration of DEX is able to induce apoptosis of immature thymocytes,and the slightly modified method of TUNEL is better.

关 键 词：地塞米松 细胞凋亡 胸腺细胞 末端标记 

分 类 号：R392.12[医药卫生—免疫学] R977.11[医药卫生—基础医学]