紫云英根瘤菌由种子浸提物诱导的基因调控片段的克隆和分析  

CLONING AND ANALYSIS OF THE REGULATORY GENE FRAGMENT INDUCED WITH SEED EXTRACT IN RHIZOBIUM HUAKUII STRAIN 7653R

作　　者：农广[1] 张忠明[1] 胡福荣[1] 陈华癸[1] 

机构地区：[1]华中农业大学农业微生物重点开放实验室,武汉430070

出　　处：《微生物学报》1998年第3期225-228,共4页Acta Microbiologica Sinica

基　　金：国家攀登计划;国家自然科学基金

摘　　要：The large plasmids of strain 7653R were digested with restriction enzyme EcoRI. Their DNA fragments were cloned into the expression vector pMP220 to construct a lacZ fusion pool, which were transferred into the recipient strain 7653R Tri-transconjugants were selected onto plates containing X-gal and seed extract Five blue colonies were assayed of their β-galactosidase activity after incubation with or without seed extract. A positive induced strain HN18 was obtained. Hybridization of nodDABC probe on the recombinant plasmid pHN18 showed a 1.7kb positive band.The evidence makes a deduction that the pHN18 containes a promoter of nod operon.The large plasmids of strain 7653R were digested with restriction enzyme EcoRI. Their DNA fragments were cloned into the expression vector pMP220 to construct a lacZ fusion pool, which were transferred into the recipient strain 7653R Tri-transconjugants were selected onto plates containing X-gal and seed extract Five blue colonies were assayed of their β-galactosidase activity after incubation with or without seed extract. A positive induced strain HN18 was obtained. Hybridization of nodDABC probe on the recombinant plasmid pHN18 showed a 1.7kb positive band.The evidence makes a deduction that the pHN18 containes a promoter of nod operon.

关 键 词：紫云英根瘤菌 种子浸提物 DIG杂交 lacZ融合 

分 类 号：Q939.114[生物学—微生物学]