丙型肝炎病毒免疫电镜的初步研究  

Preliminary Studies of Hepatitis C Virus with Immune Electron Microscopy

作　　者：王理富[1] 李德富[1] 郎淑慧[1] 尹红章[1] 冯建平[1] 

机构地区：[1]中国药品生物制品检定所卫生部生物技术产品检定及标准化重点实验室

出　　处：《中国病毒学》1998年第2期128-134,共7页Virologica Sinica

基　　金：国家"九五"科技攻关课题

摘　　要：采用组织匀浆免疫沉淀后负染、免疫组化块染后包埋、原位包埋等免疫电镜技术，研究丙型肝炎病毒（ＨＣＶ）。组织匀浆、免疫沉定、负染后在电镜下观察到与ＨＣＶ相关的类病毒颗粒，形态与披膜病毒相似，大小多在５５～６５ｎｍ，圆形，有包膜，边缘略有突起或比较平滑，有胶体金结合在此种颗粒上及其周围。无关单抗阴性对照无类似颗粒及胶体金。免疫酶染电镜下还见到成堆可疑颗粒。此外，ＨＣＶ－Ｅ区抗原染色后原位包埋，尚发现胶体金大多结合于大小５０ｎｍ左右圆形结构的内部，表明Ｅ区单抗针对的特异性抗原位点位于这种结构的内侧。With the technique of immune electron microscope,the morphology and structure of HCV were studied.After homonization of liver tissue,immune precipation with McAb to HCV C region′s antigen, and negative staining,a kind of HCV associated virus like particles was visualized.They were mainly 55-56 nm in diameter,spherical,enveloped and uneven or smooth fringe,which are similar to Togaviradae morphologically.With indirect immuno electron microscopy,there were colloidal gold particles conjugated with the virus particles and sorrounding meteriales. With immune staining of tissue piece before embed, doubious particles like virus, particles morphologically were visualized in piles. And, with in situ embed after immune staining with HCV E region′s McAb,a kind of things,which were about 50 nm in diameter,circular and conjugated with colloidal gold inside,was visualized.

关 键 词：丙型肝炎病毒 单克隆抗体 免疫电镜 

分 类 号：R373.21[医药卫生—病原生物学]