毛细管气相色谱分析昆虫病毒包涵体中的脂肪酸  

Analysis of Fatty Acids in Inclusion Bodies of Insect Viruses by Capillary Gas Chromatography

作　　者：朱湘民[1] 郑大胜[1] 汤显春[1] 夏祥明[1] 宋冬林[1] 

机构地区：[1]中国科学院武汉病毒研究所

出　　处：《中国病毒学》1998年第2期174-178,共5页Virologica Sinica

摘　　要：病毒脂肪酸的定性和定量，可作为病毒分类鉴定的辅助证据。有关细胞脂肪酸的研究已有报道［１～２］，但对病毒多角体（ｐｏｌｙｈｅｄｒｏｎｓ）和颗粒体（ｇｒａｎｕｌｅｓ）中脂肪酸成份的研究尚未见报道。本研究在病毒单糖分析的基础上［３］，用毛细管气相色谱法，对...A method for analysis of fatty acids in the insect inclusion body viruses by capillary gas chromatography was described. The main components of the fatty acid chromatogram were identified ,showing that the fatty acids in the granulosis virus and nuclear polyhedrosis virus were mainly composed of undecanoic a.,dodecanoic a.,tridecanoic a.,tetradecanoic a.,hexadecanoic a., heptadecanoic a., octadecanoic a., eicosanoic a. and octadecanoic a..Based on the ratios of the related fatty acid peak heights among distinct chromatograms,the viruses were differentiated and identified.The conclusion was consistent with that of the virus monosaccharide and pyrolysis gas chromatography.

关 键 词：核型多角体病毒 脂肪酸 毛细管气相色谱 包涵体 

分 类 号：S476.14[农业科学—农业昆虫与害虫防治]