水稻体细胞培养中胚性细胞出现与IAA的关系  

CORRELATION BETWEEN APPEARANCE OF EMBRYOGENIC CELLS AND THE IAA LEVELS IN RICE SOMATIC CELL CULTURE

作　　者：陈以峰[1,2] 周燮[1,2] 汤日圣[1,2] 张金渝[1,2] 梅传生[1,2] 

机构地区：[1]南京农业大学农学系 [2]江苏省农业科学院农业生物遗传生理研究所

出　　处：《Acta Botanica Sinica》1998年第5期474-477,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：农业部重点课题

摘　　要：水稻体细胞培养中胚性细胞出现与ＩＡＡ的关系陈以峰１周燮２汤日圣２张金渝２梅传生１（南京农业大学农学系南京２１００９５）２（江苏省农业科学院农业生物遗传生理研究所南京２１００１４）关键词水稻，胚性细胞，ＩＡＡ，２，４二氯苯氧乙酸，２，３，５三碘...Changes of endogenous IAA level and IAA action in cultured rice (Oryza sativa L.) somatic cells during the period from 7th to 15th day which was the transition from somatic to embryogenic cells were observed. The study was carried out in three experimental systems viz. mature caryopsis and young panicles (2～5 mm long) of rice cv. “Guangluai 4” under normal osmosis (3% sucrose), mature caryopses from rice cv. “Yanjing 2” or “Guangluai 4” under normal and higher osmosis (5% sucrose or 2.5% sorbitol). During this period, endogenous IAA contents were greatly increased in youngpanicle calli under normal osmosis and maturecaryoptic calli under higher osmosis but decreased in maturecaryoptic calli under normal osmosis. Exogenous IAA could induce the appearance of embryogenic cell from nonembryogenic callus at a lower frequency. And 2,3,5triiodobenzoic acid could increase the frequency of embryogenic cell induction. From these results it could be concluded that accumulation of higher IAA level in the cultured rice cells was essential for induction of embryogenic cell appearance. Since 2,4D was involved in all induction medium with the same concentration but exerted different effects on embryogenic cell induction, it was suggested that it might act through mediating the endogenous IAA metabolism.

关 键 词：水稻 胚性细胞 IAA 

分 类 号：S511.101[农业科学—作物学] Q944.46[生物学—植物学]