皖南尖吻蝮蛇蛇毒纤溶组分中结合态钙的生化特性  

Biochemical Properties of BoundCa2+ in Fibrinolytic Principle(FP) Separated from Agkistrodon acutus Venom

作　　者：王守业[1] 张祖德[1] 陈真龙[1] 余华明[1] 吴双顶[1] 刘清亮[1] 

机构地区：[1]中国科学技术大学化学系

出　　处：《生物化学与生物物理学报》1998年第3期299-302,共4页

基　　金：国家自然科学基金

摘　　要：利用原子吸收法和荧光探针法测定了皖南尖吻蝮蛇（Ａｇｋｉｓｔｒｏｄｏｎａｃｕｔｕｓ）蛇毒纤溶组分（ＦＰ）每分子中含有一个钙（Ｉ），以Ｔｂ３＋作荧光探针测得Ｔｂ３＋与ＦＰ中色氨酸（Ｔｒｐ）残基之间的距离约为０．３７５ｎｍ。It has been determined that each FP molecular contains one Ca2+binding site. By the use of fluorescence probe Tb3+, the distance between Tb3+ and tryptophan(Trp) residue was obtained to be 0.375. Tb3+ ion is coordinated with FP more strongly than Ca2+ ion, and can bind to FP and replace the Ca2+ ion in FP completely.

关 键 词：纤溶组分 荧光光谱 尖吻蝮蛇 蛇毒 结合态 钙 

分 类 号：R996.3[医药卫生—毒理学]