皖南尖吻蝮蛇蛇毒中纤溶组分的荧光特性  

Fluorescence Analysis of the Fibrinolytic Principle(FP) from Agkistrodon acutus Venom

作　　者：王守业[1] 余华明[1] 陈真龙[1] 张祖德[1] 吴双顶[1] 刘清亮[1] 

机构地区：[1]中国科学技术大学化学系

出　　处：《生物化学与生物物理学报》1998年第3期302-306,共5页

基　　金：国家自然科学基金

摘　　要：用荧光光谱方法研究了皖南尖吻蝮蛇（Ａｇｋｉｓｔｒｏｄｏｎａｃｕｔｕｓ）蛇毒中纤溶组分（ＦＰ）。研究了Ａｃｒ（丙烯酰胺）对ＦＰ内源荧光的影响，结果表明，每个ＦＰ分子含有多个Ｔｒｐ残基，这些Ｔｒｐ残基约有８３％可被Ａｃｒ所淬灭，而且这些Ｔｒｐ残基位于ＦＰ分子的较疏水环境中。同时研究了Ｃａ２＋、ＥＤＴＡ（乙二胺四乙酸二钠）、ＳＤＳ（十二烷基硫酸钠）和几种荧光淬灭剂对ＦＰ内源荧光的影响。The conformation and the properties of the fibrinolytic principle (FP) from Agkistrodon acutus venom were studied by chemical modification and fluorescence spectroscopy. Results showed that there are more than one tryptophan (Trp) residue in the FP molecule and they are located in the more hydrophobic core, could be quenched by acrylamide(Acr), a polarized quencher without electric charge. The collisional quenching constants of FP at different concentrations of Acr were calculated in terms of SternVolmer equation, and the fraction of the Trp quenched was obtained by the modified SternVolmer equation as 83%.

关 键 词：纤溶组分 色氨酸 荧光特性 尖吻蝮蛇 蛇毒 

分 类 号：R996.3[医药卫生—毒理学]