小檗胺对大鼠糖尿病性白内障模型中DNA损伤修复及致障过程的影响  

Effect of Berbamine on DNA Damage/Repair and Cataractogenesisof Diabetic Cataract Induced by STZ

作　　者：何浩[1] 何海鹰[1] 张家萍[1] 张昌颖[1] 

机构地区：[1]北京医科大学生物化学与分子生物学系

出　　处：《中国生物化学与分子生物学报》1998年第3期300-303,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金

摘　　要：利用链脲佐菌素（ＳＴＺ）引起的大鼠糖尿病性实验性白内障模型，观察小檗胺对晶状体上皮细胞ＤＮＡ损伤、修复及致障过程的影响．发现ＳＴＺ对照组在腹腔注射ＳＴＺ后３～４ｄ开始出现有显著意义的ＤＮＡ单链断裂（ｓｉｎｇｌｅｓｔｒａｎｄｂｒｅａｋｓ，ＳＳＢ），并持续存在于发病全程直至晶状体完全混浊．而在注射ＳＴＺ后１２ｈ再腹腔注射３．４８ｍｇ／ｋｇ体重，１．７４ｍｇ／ｋｇ体重小檗胺后，一周后才出现有显著意义的ＳＳＢ．３．４８ｍｇ／ｋｇ体重组在５周后白内障形成率明显低于ＳＴＺ组的同时，ＳＳＢ也恢复到对照水平，而１．７４ｍｇ／ｋｇ体重组第７周才恢复到对照水平．提示抗氧化药物小檗胺能在一定程度上阻止白内障发生过程中的ＤＮＡ损伤．The effect of berbamine,a monomer extracted from a Chinese herb (an antioxidative and a free radical scavenger),on DNA damage/repair in cataractogenesis of diabetic cataract induced by streptozotocin(STZ) was studied.The results showed that 348mg/kg b. wt. of berbamine could inhibit or delay the appearance of DNA damage (SSB) and accelerated DNA repair(as well as decrease the incidence of cataract).While 174 mg/kg b. wt. of berbamine was not as effective as that of 348 mg/kg b. wt. of berbamine.SSB appeared very early and continued into opaque phase and a positive correlation between SSB and lens opacity was showed.Oxidative damage may be one of the most important mechanisms in diabetic cataractogenesis.Berbamine has the preventive effect on cataractogenesis.

关 键 词：DNA损伤 小檗胺 糖尿病 白内障 病理学 

分 类 号：R587.2[医药卫生—内分泌] R276.761[医药卫生—内科学]