失血性休克后IL-1活性变化及其与肠源性内毒素血症的关系  

ALTERATIONS OF IL 1 ACTIVITY AND ITS RELATION WITH ENTEROGENOUS ENDOTOXEMIA IN HEMORRHAGIC SHOCK

作　　者：石汉平[1] 齐德林[1] 盛权根[1] 

机构地区：[1]第一军医大学南方医院普外科

出　　处：《中国应用生理学杂志》1998年第2期154-156,共3页Chinese Journal of Applied Physiology

摘　　要：目的：观察失血性休克后血浆ＩＬ－１活性的变化及其与肠源性内毒素血症的关系。方法：复制容量控制失血性休克及肠源性内毒素血症模型，改良ＬＡＦ法及显色基质偶氮法检测ＩＬ－１及ＥＴ。结果：普通ＳＤ大鼠３０％失血后２～３ｈ及６～１０ｈ血浆ＩＬ－１活性升高，３ｈ后ＥＴ水平显著升高；失血前胃内灌注双岐杆菌或失血后静注ｒＢＰＩ，大鼠血浆ＥＴ水平无明显升高，ＩＬ－１活性第１峰仍然存在，但第２峰消失。无菌ＳＤ大鼠１０％失血、灌注ＬＰＳ后１ｈ血浆ＥＴ水平升高，２ｈ后ＩＬ－１活性升高。结论：失血性休克后ＩＬ－１活性呈双峰型升高，第１峰与内毒素无关，第２峰为内毒素刺激峰。Objective: To observe alterations of IL 1 activity and its relation with enterogenous endotoxemia in hemorrhagic shock. Method: Plasma IL 1 activity and endotoxin (ET) level were tested with modified LAF method and limulus test respectively in reproducing volume controlled hemorrhagic shock and enterogenous endotoxemia model. Result: There were significant increases in plasma IL 1 activities during 2～3 h and 6～10 h, and in plasma ET level during 3～10 h in conventional SD rats posthemorrhagic shock. After oral administration of bifidobacteria prehemorrhage or introvenous injection of rBPI posthemorrhage, there was a marked increase in plasma IL 1 activity during 2～3 h posthemorrhage, no remarkable change was found in plasma ET level during 3～10 h, or plasma IL 1 activity during 6～10 h posthemorrhage. While there were significant increases in plasma ET level at 1h and in plasma IL 1 activity at 2 h in endotoxemia rats after lipopolysaccharide (LPS) administration. Conclusion: Plasma IL 1 activity rise as a two peak style post hemorrhagic shock. The first peak (2～3 h posthemorrhage) has no relation with ET. While the second peak (6～10 h posthemorrhage) is caused mainly by ET which is derived mainly from guts.

关 键 词：失血性休克 白细胞介素1 肠源性 内毒素血症 

分 类 号：R605.971[医药卫生—急诊医学] R515.302[医药卫生—外科学]