三羟异黄酮对丙烯酰胺致小脑神经元凋亡的影响  

The effect of genistein on cerebellar neuronal apoptosis induced by acrylamide

作　　者：江城梅[1] 赵文红[1] 赵红[1] 申玲[1] 王丹妮[1] 

机构地区：[1]蚌埠医学院预防医学系,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2009年第1期1-4,共4页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究项目资助项目(2006kj345B)

摘　　要：目的:研究三羟异黄酮对丙烯酰胺引起小脑神经元细胞凋亡的影响。方法:建立丙烯酰胺诱导的小脑神经细胞凋亡模型,观察自主性行为;测定氧化应激功能指标,谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化脂质(MDA),电镜观察神经元细胞的变化,并观察三羟异黄酮的保护作用。结果:染毒组自主性行为的活动次数明显减少。生化指标染毒组大鼠的小脑组织中GSH、SOD与对照组比较含量减少(P<0.01),MDA含量增加(P<0.01)。电镜下,神经元细胞水肿,线粒体空泡样改变,出现凋亡小体,三羟异黄酮(50、100 mg.kg-1.d-1)能够使GSH、SOD含量增加,MDA减少,细胞形态结构损伤明显减轻。结论:三羟异黄酮能够抑制丙烯酰胺诱导的小脑神经细胞凋亡。Objective： To investigate the effect of genistein on the apoptosis of cerebellar neuronal cells induced by acrylamide. Methods：The model of cerebellar neuronal apoptosis in rats was established by intragastric administration of acrylamide, and their independence behaviour was observed, the index of oxidative stress：GSH-PX, SOD and MDA was determined,the changes of cerebellar neuronal cells was observed electron microscopy. In the meantime, the protective effect of Einstein was observed. Results： The frequency of voluntary movements in rats of the group treated with acrylamide was significantly lower than that of control group（ P 〈 0.01 ）. The contents of GAH-PX, SOD of cerebellar tissue of the group treated with acrylamide were significantly lower than that of control group（P 〈 0.01 ） and the contents of MDA was much higher than that of control group（ P 〈 0.01 ）. The edema of cerebellar cells, the vacuolar degeneration of plastochondria and the apoptotic bodies were observed under electron microscope. Treatment with 25 mg·kg^-1·d^-1 and 50mg·kg^-1·d^-1 of genistein increased the contents of GAH-PX and SOD, and reduced the content of MDA and the injury of cerebellar cells. Conclusions ：The results indicate that genistein can reduce cerebellar neurocyte apoptosis induced by acrylamide.

关 键 词：三羟异黄酮 丙烯酰胺 自主性行为 氧化应激指标 小脑神经 细胞凋亡 

分 类 号：R977.12[医药卫生—药品]