前列腺素E1脂微球制剂对大鼠急性肺损伤的影响  

Effects of lipo-PGE1 on Acute Lung Injury induced by LPS in Rats

作　　者：李湘灵 姜远旭 

机构地区：[1]湖北省黄石市二医院麻醉科,黄石435000

出　　处：《数理医药学杂志》2009年第1期24-26,共3页Journal of Mathematical Medicine

摘　　要：目的:采用大鼠腹腔注射脂多糖(LPS)复制大鼠急性肺损伤(ALI)模型,评价前列腺素E1脂微球制剂(凯时)对急性肺损伤大鼠的保护作用。方法:雄性健康Sprague Dawley大鼠60只随机分为3组,A组:生理盐水组;B组:LPS模型组;C组:凯时治疗组。动物以硫喷妥纳腹腔注射麻醉,于实验3h分别测量每只大鼠的肺系数,肺通透指数,肺湿重/肺干重,肺组织中髓过氧化物酶(MPO)含量,丙二荃(MDA)和过氧化物歧化酶(SOD)的含量,血清细胞因子TNF-a、IL-12、IL10的浓度,肺组织中NF-KB蛋白的表达,并进行统计学分析。结果:①B组与A组比较,肺系数,肺通透指数,肺湿重/肺干重显著增加(P<0.05);血浆SOD含量,血清细胞因子IL10显著减少(P<0.05);肺组织中髓过氧化物(MPO)活性和丙二荃(MDA)含量,血清细胞因子TNF-a,IL-12显著增加(P<0.05)。②C组与B组比较,肺系数,肺通透指数,肺湿重/肺干重显著减少(P<0.05);肺组织SOD含量,血清细胞因子IL10显著升高(P<0.05);肺组织中髓过氧化物(MPO)活性和丙二荃(MDA)含量,血清细胞因子TNF-a,IL-12显著减少(P<0.05)。③C组与B组比较,NF-kB P65的表达明显低于A组(P<0.05)。结论:前列腺素E1脂微球制剂通过抑制NF-kB的表达,减少中性粒细胞的渗出,对LPS诱导的急性肺损伤大鼠有明显保护作用。Objective： To investigate the protective effects of lipo-PGE1 on acute lung injury injury induced by lipopolysaccharied （LPS） in rats. Methods： In a All model by injected LPS through abdominal cavity,Sprague Dawley rats weighing 180g-220g were randomly devided into 3 groups with twenty each： group A（normal group,n=20）,group B （LPS group,n=20）, group C（LPS＋lipo-PGE group）. Lung index.Pulmonary microvasular permeability index, Wet/Dry weight, MPO, MDA, SOD, TNFA, IL12, IL10 were measured and statistically analyzed. Results： （1） As compared with the normal group. Lung index, Pulmonary microvasular permeability index, Wet/Dry weight increased significantly （P〈0.05）; SOD, IL-10 level decreased singnificantly （P〈0. 05）; MPO, MDA, SOD, TNFA, IL12 increased singnificantly （P〈0. 05）in the acute lung injury group. （2） Lung index, Pulmonary microvasular permeability index, Wet/Dry weight were significantly lower（P〈0. 05） ; SOD, IL10 level were significantly higher（P〈0. 05） ; MPO, MDA, SOD, TNFA, IL12 were markedly lower（P〈0.05）in lipo-PGE1 group than those in LPS-inducde group. （3） A significant decrease was in the phosphorylation of Ser536 on NF-kB observed in the lipo-PGE1 group, compared with the acute lung injury grou（P〈0. 05）. Conclusion： lipo-PGE1 could inhibit expression of NF-kB and reduce exudation of PMNs,and had protective effects on acute lung injury induced by LPS in rats.

关 键 词：急性肺损伤 前列腺素E1脂微球制(lipo-PGE1) 

分 类 号：R563[医药卫生—呼吸系统]