CQ11逆转多药耐药人胃癌细胞株对多柔比星的耐药  

Reversal of CQ11 on Resistance of Doxorubicin in Multidrug-resistanct Human Gastric Cancer Cells

作　　者：吴达龙[1] 睢凤英[1] 张成文[1] 吕焕章[2] 

机构地区：[1]浙江省嘉兴学院医学院药理学教研室,嘉兴314001 [2]北京市军事医学科学院附属医院药理科

出　　处：《数理医药学杂志》2009年第1期67-69,共3页Journal of Mathematical Medicine

基　　金：嘉兴市科技局科研基金(No.2007AY2033);嘉兴学院科研基金(No.70107032)

摘　　要：目的:探讨氯喹衍生物CQ11对耐长春新碱(vincristine,VCR)人胃癌多药耐药(multidrug resistance,MDR)细胞株SGC7901/VCR的耐药逆转作用。方法:将SGC7901和SGC7901/VCR细胞分别与各种浓度的多柔比星(doxorubicin,DOX)和/或CQ11在体外共同培养,采用MTT法检测其细胞毒作用;采用荧光分光光度计测定细胞内DOX蓄积量。结果:SGC7901/VCR细胞对DOX的耐药程度是SGC7901细胞的37.5倍。1.0、2.5和5.0 mol/L的CQ11分别使DOX对SGC7901/VCR细胞的敏感性分别增加到2.2倍(P<0.01)、5.5倍(P<0.01)和14倍(P<0.01)。DOX蓄积实验表明,CQ11能显著增加SGC7901/VCR细胞内DOX蓄积,而对SGC7901细胞内DOX蓄积无明显影响。结论:通过增加细胞内DOX蓄积量,CQ11在体外能有效逆转SGC7901/VCR细胞对DOX的耐药性。Objective. To investigate the reversal effect of CQ11, a chloroquine derivative, on multidrug resistance（MDR） in vincristine （VCR）-resistant human gastric carcinoma cell line SGC7901/VCR. Methods. human gastric cancer cell line, SGC7901, and its VCR-resistant variant, SGC7901/VCR, were cultivated with CQ11 and/or doxorubicin （DOX）. The cytotoxicity of drugs in vitro was assayed by MTT method. The accumulation of DOX in these cells was detected by fluorescence spectrophotometer. Results. SGC7901/ VCR cells were 37. 5 times more resistant to DOX in comparison with SGC7901 cells. At the concentrations of 1.0, 2.5 and 5.0 mol/L, CQll increased the DOX sensitivity by 2.2-fold （P〈0.01）, 5. 5-fold （P〈0.01） and 14-fold （P（0.01）, respectively. In the intracellular accumulation study of DOX, simultaneous incubation of SGC7901/VCR cells with CQ11 significantly increased the DOX accumulation in SGC7901/VCR cells. No such results were found in parental SGC7901 cells. Conclusion. CQ11 had strong in vitro MDR reversal effects by increasing intracellular DOX accumulation.

关 键 词：多药耐药 氯喹 胃癌 逆转剂 

分 类 号：R962[医药卫生—药理学]