禾本科植物内生真菌研究8——中国部分地区拂子茅属植物Neotyphodium属内生真菌分布及形态学特征  

Grass endophyte researches 8——Distribution and morphological characteristics of Neotyphodium sp. grown in Calamagrostis spp. in China

作　　者：詹漓晖[1] 纪燕玲[1] 于汉寿[1] 亢燕[1] 孙相辉[1] 王志伟[1] 

机构地区：[1]南京农业大学生命科学学院微生物学系,江苏南京210095

出　　处：《草业科学》2009年第1期13-18,共6页Pratacultural Science

基　　金：国家自然科学基金(30670008)

摘　　要：调查了中国西北和东部地区不同生境的拂子茅属Calamagrostis植物中Neotyphodium属内生真菌的分布。采样范围包括新疆、青海、甘肃、江苏、安徽、浙江、上海7省(市、区),共采集拂子茅属植物样品426株。调查发现西北3省(区)所有276个拂子茅属植物样品中都不含有内生真菌;而采集自东部的样品中,南京的无性系拂子茅属植物和黄山的拂子茅C.epigeios含菌率为100%。但是,采自南京抽穗的拂子茅却完全不含内生菌。通过研究分离菌株的形态学特征,发现南京与黄山的菌株都具有Neotyphodium属内生真菌的典型特征,而它们之间在菌落形态、生长速度、孢子大小等方面又存在较明显差异。以上结果说明我国拂子茅属植物及其中的禾本科植物内生真菌具有一定的生物多样性,拂子茅属植物中的内生真菌含量受地理环境以及宿主植物种类的影响十分明显。The distribution of Neotyphodium spp. grown in Calarnagrostis spp. in the northwest and east parts of China was investigated from 2006 to 2007. Totally 426 of Calarnagrostis spp. plants were collected from Xinjiang, Qinghai, Gansu, Jiangsu, Anhui, Zhejiang and Shanghai. In which, 276 samples from the west of China were endophyte free, while non-inflorences Calarnagrostis sp. in Nanjing and C. epigeios in Huangshan were 100% infected by endophyte. In contrast, C. epigeios in heading period in Nanjing was endophyte free. Morphological properties of isolates were observed and these isolates were different in colony morphology, growth rate and conidia size. Neotyphodiurn spp. in native Calamagrostis spp. showed abundant diversity. The level of endophyte concentration varied with geographical distribution and host species.

关 键 词：内生真菌 拂子茅属 地理分布 形态学特征 多样性 

分 类 号：S182[农业科学—农业基础科学]