HBV C基因启动子变异及基因型与肝硬化及原发性肝癌的相关性研究  

Mutations in the core promoter of hepatitis B virus and viral genotype are correlated with hepatocellular carcinoma

作　　者：侯旭芬 张汉荣[2] 刘新钰[2] 孙梅[2] 

机构地区：[1]江苏省靖江市肿瘤医院,江苏靖江214515 [2]江苏省南京市第二医院,江苏南京210003

出　　处：《海南医学》2009年第2期1-3,共3页Hainan Medical Journal

摘　　要：目的探讨乙型肝炎病毒C基因启动子(CP)和前C区基因变异及HBV基因型与肝硬化及原发性肝癌(HCC)的关系。方法通过DNA扩增、基因序列分析检测44例慢性乙型肝炎(CH),39例活动性肝硬化(LC),34例HCC患者血清HBVS基因序列,确定其基因型,测定HBV CP和前C区序列,确定其变异状况。结果HCC、LC患者CP双变异(nt1762A→T和1764G→A)发生率(82.4%、71.8%)显著高于CH患者(45.5%)(P<0.01、P<0.01);HCC、LC患者C基因型检出率(70.6%、69.2%)显著高于CH患者(43.2%)(P值均<0.05)。结论乙型肝炎病毒CP双变异、C基因型与HCC、LC密切相关。Objective To study the relationship of mutations in the core promoter（CP） and viral genotype with hepatocellular carcinoma（HCC）. Metheds S region, CP were sequenced directly from sera of 44 cases of patients with chronic hepatitis B（ CH）, 39 patients with liver cirrhosis （LC） and 34 cases of patients with HCC after amplication by the polymerase chain reaction. Results CP mutations in CP（ A - to - T an nt 1762 and G - to - A at nt 1764） were observed in 21 of 44 cases of patients with CH（45.5% ）, 27 of 39 cases of patients with LC （71.8%） and 24 of 34 cases of patients with HCC（82. 4% ）. There was significant difference in the prevalence of CP mutations between patiens with CH, LC and HCC. Genotypes B and C occrred in 47 （40.2%） and 70 （59. 8% ） of samples. Patients with LC and HCC has a higher prevalence of genotypes C than patients with chronic hepatitis B （69. 2%, 70. 6% vs 43.2%, P 〈 0.05 ）. Conclusion T1762/A1764 mutations in CP increse the risk of HCC,LC. Genotype C is closely correlated with the development of LC and HCC.

关 键 词：肝炎病毒 乙型 基因型 变异 肝硬化 聚合酶链式反应 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学]