细茎大豆(G.gracilis)rbcS基因结构与分子进化分析  

The Structure and Evolution Analysis of G. gracilis rbcS Gene

作　　者：张二荃[1] 王喜萍[1] 曹凯鸣[1] 顾其敏[1] 庄炳昌[2] 

机构地区：[1]复旦大学 [2]吉林省农科院大豆所

出　　处：《复旦学报（自然科学版）》1998年第2期151-156,共6页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金

摘　　要：从细茎大豆的嫩叶中提取总ＤＮＡ，用ＰＣＲ方法扩增得到包含完整编码区的 ｒｂｃ Ｓ基因并将其克隆到ＰＢＬＵＥＳＣＲＩＰＴ载体中．完成全基因 １０８９个核苷酸测序后，运用ＰＣ－ＧＥＮＥ进行顺序编辑和同源比较，并应用ＭＥＧＡ１．０２软件中的Ｎｅｉｇｈｂｏｒ－Ｊｏｉｎｉｎｇ方法画出Ｒｕｂｉｓｃｏ小亚基分子的系统进化树．Total DNA was extracted from tender leaves of semi-cultural soybean (G. gracilis). The rbcS containing the whole coding region for Rubisco small subunit was amplified by PCR and then cloned and sequenced. This gene was subjected to homology analysis from PCGENE package, and the phylogenetic tree of Rubisco small subunit from different plants was constructed by neighbor-joining method of MEGA 1. 02 package.

关 键 词：大豆 序列分析 分子进化 rbcs基因 细茎大豆 

分 类 号：S565.101[农业科学—作物学] Q949.751.9[生物学—植物学]