核酶的化学合成及其对烟草花叶病毒RNA片段的体外剪切  

Chemical Synthesis of Ribozyme and Its Cleavage Activity to TMV-RNA in vitro

作　　者：金春阳[1] 杨建华[1] 岳颖[1] 杨再完[2] 于善谦[1] 章道道[1] 

机构地区：[1]复旦大学 [2]中国科学院上海有机化学研究所

出　　处：《复旦学报（自然科学版）》1998年第2期191-196,共6页Journal of Fudan University：Natural Science

基　　金：国家科委攀登计划;国家自然科学基金;中国科学院上海有机化学研究所生命有机国家重点实验室资助课题

摘　　要：设计并用化学方法合成了针对烟草花叶病毒ＲＮＡ的核酶Ｒｚ－１及Ｒｚ－１小型化的核酶Ｒｚ－２以及它们的底物ＲＮＡ片段Ｓｂ．研究了两种核酶对底物Ｓｂ的体外催化活性，并把核酸Ｒｚ－１与生物合成的相同组成的核酶Ｒｚ－１’进行了比较试验．The ribozyme Rz-1,minimized ribozyme Rz-2 which was modified from Rz-1 and their substrate Sb,a target RNA fragment of tobacco mosaic virus were designed and synthesized by chemical method. The results indicated that both the Rz-1 and Rz-2 showed cleaving activities on their substrates in vitro. The cleaving activity of ribozyme Rz-1 was also compared with that of ribozyme Rz-1' which was synthesized by transcription.

关 键 词：核酶 烟草花叶病毒 体外剪切 化学合成 RNA片段 

分 类 号：Q520.3[生物学—生物化学] S432.41[农业科学—植物病理学]