对大鼠PC12细胞用于定量测定神经生长因子活性两种方案的评价  

Evaluation of Two Protocols for Quantitative Bioassay of Nerve Growth Factor Activity with Rat Pheochromocytoma PC12 Cells

作　　者：林万敏[1] 凌文海[1] 刘建平[1] 张卓兵 殷明[3] 谢毅[1] 

机构地区：[1]复旦大学 [2]烟台荣昌制药有限公司 [3]海军医学研究所

出　　处：《复旦学报（自然科学版）》1998年第2期218-220,共3页Journal of Fudan University：Natural Science

基　　金：烟台荣昌制药有限公司资助课题

摘　　要：建株的大鼠肾上腺嗜铬细胞瘤ＰＣ１２细胞，在神经生长因子ＮＧＦ的作用下，会长出神经突起．这一现象被用于神经生长因子的活性测定．但就定量测定而言，目前已报道的方法、方案、包括判定阳性细胞的标准，均有一定差异，而我国也尚未建立起对这一重要分子活性的标准测定法．通过比较两种不同的方案，提示高浓度的神经生长因子先行对细胞进行长时间的预处理并非为测定所必需；同时提出了比较简便可行的准则，包括判定阳性细胞的标准，对方法的不足也进行了讨论．Cultured rat pheochromocytoma PC12 cells, exposed to nerve growth factor (NGF), can form neurite processes. This phenomenon has been used for NGF bioassay. However the protocols reported for quantitative assay of NGF, including the criteria for judging positive cells, are various. In China the standard method for measuring this important molecule has not been established yet. In this study, two different protocals were compared, suggesting that long-period pretreatment of PC12 cells with concentrated NGF was not of prerequisite for quantitative assay of NGF activity. This simple and easily-performed protocal was recommended and its shortage was discussed.

关 键 词：PC12细胞 神经生长因子 定量测定 神经系统 活性 

分 类 号：R338[医药卫生—人体生理学] R971[医药卫生—基础医学]