高效液相色谱法测定刺五加注射液中异秦皮啶含量  

Determination of Isofraxidin in Ciwujia Injection by HPLC

作　　者：金佩芬[1] 

机构地区：[1]浙江省嘉兴市药品检验所,浙江嘉兴314001

出　　处：《中国药业》2009年第3期9-9,共1页China Pharmaceuticals

摘　　要：目的建立刺五加注射液中异秦皮啶的含量测定方法。方法使用C18柱(150mm×4.6mm,5μm),以乙腈-0.1%磷酸溶液(20:80)为流动相,检测波长为344nm。结果进样量与峰面积线性关系良好,异秦皮啶平均回收率为99.69%,RSD为1.33%(n=6)。结论高效液相色谱法分离良好,可用于刺五加注射液的质量控制。Objective To establish the determination method of the isofraxidin content in Ciwujia Injection. Methods HPLC was used to determine isofraxidin in Ciwujia Injection on C18 column（150 mm×4.6 mm, 5 μm）, with acetonitrile-0. 1% phosphoric acid solution（20： 80） as the mobile phase and the detection wavelength of 344 nm. Results The linearity of this method was better. The average recovery rate was 99.69%, RSD was 1.33% （n=6）.Conclusion This method shows good separating degree and can be used for the quality control of Ciwujia Injection.

关 键 词：高效液相色谱法 异秦皮啶 刺五加注射液 含量 

分 类 号：R927.2[医药卫生—药学] R979.1