复方电解质注射液作添加液汇集血小板的研究  

In Vitro Evaluation of Platelet Concentrates from Pooled Buffy Coats in Multiple Electrolytes Injection

作　　者：黄成垠[1] 袁克宇[1] 肖建宇[1] 蒋昵真[1] 史广耀[1] 蔡莉[1] 魏鹏[1] 唐荣才[1] 

机构地区：[1]江苏省血液中心,南京210042

出　　处：《临床输血与检验》2009年第1期6-9,共4页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：江苏省卫生厅科研基金项目(No.H200622)资助

摘　　要：目的探讨复方电解质注射液作添加液(PAS)汇集多人份混合血小板的可行性。方法从400ml全血中分离白膜层(BC),容量40～45ml,于22℃±2℃静置过夜,将ABO同型的6袋白膜汇集,加200ml复方电解质注射液稀释白膜,稀释后的白膜在温度22℃±2℃的离心机中,以900r/min离心10min,上层富含血小板悬液经白细胞过滤器去除白细胞,并转移到血小板保存袋内,即制备成1个成人治疗量的PAS汇集BC-PCs。结果共制备10个成人治疗量的PAS汇集BC-PCs,其容量、血小板含量、WBC混入量、RBC混入量分别为:(293±22)ml、(3.01±0.29)×1011、(1.1±0.2)×106、(5.9±1.3)×109。保存8d后的pH、低渗休克反应率(HSR)、形变能力(ESC)、CD62P表达率、AnnexinV结合率分别为7.10±0.05、(65.6±7.1)%、(7.1±1.6)%、(27.4±3.3)%、(12.0±1.4)%。结论复方电解质注射液作为添加液汇集血小板的方法可行。Objective To develop a method to produce platelet concentrates （PCs） from pooled huffy coats in additive solution （muhiple electrolytes injection）. Methods PCs were made from six pooled huffy coats （12 whole-blood units）and 200 ml muhiple electrolytes injection. After eentriftlgation the PCs were leukoreduced with a filter and stored in a 600 ml platelet storage bag. PCs were sampled on days 1, 3, 5 and 8 of storage and tested in vitro quality. Results Routinely produced PCs （n=10） contained （3. 01±0. 29）×10^11 platelets in volume of （293±22） mh The WBC and RBC count were （1.1 ±0. 2）× 10^6 and （5.9±1. 3）× 10^9 per PCs. The in vitro quality of the PCs was maintained for up to 8 days. The pH, hypotonic shock response（HSR）,extent of shape change（ESC）,CD62P expression and AnnexinV binding of PCs after 8^th day of storage were 7.10±0.05,（65.6±7.1）%,（7.1±1.6）%, （27. 4± 3. 3） % and（12.0±l.4）%. Conclusion The method of preparing PCs from pooled huffy coats in muhiple electrolytes injection is feasible in terms of the in vitro quality of PCs.

关 键 词：添加液 复方电解质溶液 浓缩血小板 汇集白膜层法 

分 类 号：R331.43[医药卫生—人体生理学]