利用十二肽库筛选心肌型脂肪酸结合蛋白特异性的亲和配体  

Scanning Affinity Ligand of Heart-type Fatty Acid Bound Protein with Phage-displayed 12-mer Peptide Library

作　　者：张淑华[1,2] 王立成[1] 王丽萍[1] 于源华[2] 李惟[1] 

机构地区：[1]吉林大学生命科学学院,长春130021 [2]长春理工大学生命科学技术学院,长春130022

出　　处：《吉林大学学报（理学版）》2009年第1期140-143,共4页Journal of Jilin University:Science Edition

基　　金：国家高技术研究发展计划863项目基金(批准号:2007AA032314)

摘　　要：采用噬菌体表面展示十二肽库对心肌型脂肪酸结合蛋白(H-FABP)特异性亲和配体进行筛选,经3轮筛选及序列比对后得到一个酶联免疫测定分析(ELISA)检测阳性特征序列:W-P-N-H-H-M-L-H-K-R-W-P.对此序列进行肽合成,并标记上荧光标记物芘,经高效液相纯化、冻干及质谱确定其分子量后制成荧光肽探针检测急性心肌梗塞病人血样,结果均呈阳性.结果表明,所获得的肽探针具有较好的临床应用效果.We obtained the specific sequence of affinity ligand of H-FABP with phage-display 12-mer peptide library. After three rounds of panning and sequences comparison, we obtained one ELISA positive sequence of W-P-N-H-H-M-L-H-K-R-W-P. The target peptide was then synthesized by manual way. Treated by reverse phase HPLC （High Pressure Liquid Chromatography） and mass spectrometry, the target peptide coupled with pyrene was used as a fluorescent probe to detect H-FABP in the samples of AMI patients. The experiment result indicates that the samples of AMI patients are all positive. The result reflects that the target ligand peptide we got has shown a good effect on clinical application for AMI.

关 键 词：心肌型脂肪酸结合蛋白 噬菌体表面展示十二肽库 亲和配体 酶联免疫测定分析 

分 类 号：Q513[生物学—生物化学]