高效液相色谱法测定上清丸中大黄素和大黄酚的含量  

Determination of emodin and chrysophanol in Shangqing pills by HPLC

作　　者：肖燕[1] 刘建锋[2] 

机构地区：[1]怀化市药品检验所,湖南怀化418000 [2]中南大学药学院,长沙410013

出　　处：《中南药学》2009年第1期39-42,共4页Central South Pharmacy

摘　　要：目的建立上清九中大黄素和大黄酚的含量测定方法。方法色谱柱为Diamonsil C18（250mm×4．6mm，5μm），流动相为甲醇-0．1％磷酸溶液（85：15），流速：1．OmL·min^-1，检测波长：254nrn。结果大黄素进样量在0．0576～0．1536μg线性关系良好，r=0．9999，平均加样回收率为98．49％，RSD为1．75％（n=6）；大黄酚进样量在0．1397～0．3725μg线性关系良好，r=0．9998，平均加样回收率为98．86％，RSD为1．24％（n=6）。结论该方法操作简便，结果准确，重现性好，适用于上清丸中大黄素和大黄酚的含量测定。Objective To establish the content determination method of emodin and chrysophanol in Shangqing pills. Methods Diamonsil C18 （250 min×4. 6 mm, 5 μm） was used. The mobile phase was methanol-0.1%Phosphorylation （85 ： 15） with a flow rate of 1.0 mL · min^-1. The detection wavelength was 254 nm. Results A good linearity was obtained for emodin at 0. 057 6-0. 153 6μg （r=0. 999 9）, and the average recovery was 98. 49% （RSD= 1.75%, n=6） . A good linearity was obtained for chrysophanol at 0. 139 7-0. 372 5/zg （r=0. 999 8）, and the average recovery was 98. 86% （RSD=1. 24%, n=6）. Conclusion The method is simple, accurate, and reproducible, which can be used for the determination of emodin and chrysophanol in Shangqing pills.

关 键 词：高效液相色谱法 上清丸 大黄素 大黄酚 含量测定 

分 类 号：R927.2[医药卫生—药学] R284.2