HPLC数字化指纹图谱控制甘草质量  

Quality control of RADIX ET RHIZOMA GLYCYRRHIZAE by digitized HPLC fingerprints

作　　者：孙国祥[1] 王佳庆[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中南药学》2009年第1期51-54,共4页Central South Pharmacy

基　　金：国家自然科学基金重大研究计划课题(90612002)

摘　　要：目的建立了不同产地甘草HPLC数字化指纹图谱的质量控制方法。方法采用反相HPLC法以色谱指纹图谱指数F为目标函数优化选择指纹图谱检测条件,用"中药色谱指纹图谱超信息特征数字化评价系统3.0"软件评价并建立其数字化指纹图谱。结果以甘草酸(Glycyrrhizin,GHIA)为参照物峰,确定52个指纹峰,获得了判别其质量的重要数字化信息,并以双定性双定量相似度法评价不同产地甘草质量稳定性。结论所建立HPLC数字化指纹图谱可清晰反映甘草药材的质量。Objective To establish a digitized HPLC fingerprint of RADIX ET RHIZOMA GLYCYRRHIZAE （RRGH）. Methods The RP-HPLC condition was optimized by the chromatographic fingerprint index （F）. The fingerprints were characteristically digitized and evaluated by the software of the Digitized Evaluation System for Super-information Characteristics of Traditional Chinese Medicine Fingerprints which was invented by SUN Guo-xiang. Results Fifty-two common peaks were selected as the fingerprint peaks when taking GHIA as the referential peak. The stabilities among different areas of samples were evaluated by the dual qualitative and dual quantitative similarity method. Conclusion The fingerprints can clearly and perfectly reflect the authentical quality of RRGH.

关 键 词：甘草 HPLC指纹图谱 双定性相似度 双定量相似度 双参照物指纹体系 

分 类 号：R284[医药卫生—中药学] O234[医药卫生—中医学]