雷公藤胚性愈伤组织再生植株的增殖及其稳定性  

Proliferation and Stability of the Regenerated Plantlets from the Embryogenic Callus of Tripterygium wilfordii

作　　者：李琰[1,2] 冯俊涛[1] 陈新雨[2] 毛圆辉[2] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心陕西省生物农药工程技术研究中心,杨凌712100 [2]西北农林科技大学生命科学学院,杨凌712100

出　　处：《林业科学》2009年第1期57-61,I0001,共6页Scientia Silvae Sinicae

基　　金：国家"十五"攻关项目(2002BA516A04)

摘　　要：以雷公藤胚性愈伤组织为材料,研究继代培养时间对植株再生和基本培养基、生长素浓度及其与细胞分裂素组合对再生苗增殖的影响。结果表明:在继代培养过程中,愈伤组织再生率和再生苗数经历了一个由低到高再到低的过程,以8个月到1年之间的胚性愈伤组织的再生能力最强,达100%,每块愈伤组织平均形成的芽数也比较多,雷公藤胚性愈伤组织在继代20个月后已经丧失再分化能力。愈伤组织再分化过程易发生变异,从叶形上看,再生苗出现6种类型的变化。再生苗茎段增殖以1/2MS为基本培养基附加2.0mg·L-1IBA为好,以1/2MS+2.0mg·L-1IBA+0.5g.L-1活性炭的培养基有利于芽苗生根培养,生根率达100%。移栽至珍珠岩与腐殖质土(1∶1)的混合基质中成活率达96%。The embryogenic callus of Tripterygium wilfordii was used to study the effect of its subculture duration on the plant regeneration, and the effect of basic medium with different auxin concentrations and their combination with cytokinin on the proliferation of its regenerated plantlets. The results showed that during the process of subculture of the embryogenic callus, the regeneration rate and the number of the regenerated plantlets increased in the first stage and then declined in the second stage. The embryogenic callus with its subculture age between 8 month and 1 year old was most regenerative, with 100% regeneration rate and more plantlet number per callus. The callus had lost its ability to differentiate completely after 20 months of subculture age. The differentiated plantlets from calli were vulnerable to mutate. The plantlets could be divided into 6 types, according to the shapes of leafs of the plandets. When the plantlets were propagated with stem sections, 1/2MS supplemented with 2.0 mg· L^- 1 IBA should be optimal. And 1/2MS ＋ 2.0 mg· L^- 1 IBA ＋ 0.5 g· L^- 1 active carbon medium was favorable for the plantlets to root, with rooting rate 100%. When transplanted in the mixture of perlite and humus （1：1） soil, its survival rate was as high as 96%.

关 键 词：雷公藤 胚性愈伤组织 组织培养 快繁 

分 类 号：S722.37[农业科学—林木遗传育种] Q943.1[农业科学—林学]