盾叶薯蓣中新甾体皂苷的研究  

Isolation and structure determination of steroidal saponin from Dioscorea zingiberensis

作　　者：徐德平[1] 胡长鹰[2] 王琳[1] 王啸春[1] 庞自洁[3] 

机构地区：[1]江南大学食品学院,江苏无锡214036 [2]暨南大学食品科学与工程系,广东广州510632 [3]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《药学学报》2009年第1期56-59,共4页Acta Pharmaceutica Sinica

基　　金：广东省珠海市科技局(PC20071044);江苏省发改委资助

摘　　要：为了研究盾叶薯蓣的水溶性化学成分,用溶剂提取、色谱分离等方法从盾叶薯蓣的水溶液中分离到一个新甾体皂苷,经1HNMR、13CNMR、135DEPT、HMQC、HMBC和TOCSY谱鉴定,该化合物的结构为:(25R)-26-O-(β-D-glucopyranosyl)-furost-5-en-3β,16,20,26-tetraol-22-seco-3-Ο-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside,命名为zingiberenin F(1)。该化合物为一新甾体皂苷化合物,该骨架甾体皂苷在薯蓣科中为首次报道。To study the chemical constituents of Dioscorea zingiberensis Wright, the EtOH extract of fresh rhizomes of D. zingiberensis was concentrated and partitioned further to produce petroleum ether-, ethylacetate-, n-butanol- and water-soluble fractions. The water-soluble fraction was subjected to column chromatography on macro resin AB-8, and the final products were obtained by repeated reversed-phase ODS and MCI gel CHP 20P column chromatography. Structures of compounds were elucidated by ^1H NMR, ^13C NMR, ^135DEPT, HMQC, HMBC and TOCSY spectroscopic analyses. A new steroidal saponin was isolated, which was identified as （25R）-26-O-（β-D-glucopyranosyl）-furost-5-en-3β, 16, 20, 26-tetraol-22-seco-3-O-β-D-glucopyranosyl-（1→3）-β-D- glucopyranosyl-（1→4）- [ α-L-rhamnopyranosyl-（1→2）]-β-D-glucopyranoside. The compound is a novel skeletally steroidal saponin, named as zingiberenin F （1）. It was reported for the first time from D. zingiberensis Wright.

关 键 词：盾叶薯蓣 甾体皂苷 zingiberenin F 

分 类 号：R284.2[医药卫生—中药学]