鸡肝散提取物在大鼠体内的吸收和分布  

Absorption and distribution of extracts of Elsholtzia blanda in rats

作　　者：应景艳[1,2] 王锦磊[1] 姚彤炜[1] 

机构地区：[1]浙江大学药学院,浙江杭州310058 [2]宁波市鄞州第二医院,浙江宁波315100

出　　处：《药学学报》2009年第1期69-73,共5页Acta Pharmaceutica Sinica

基　　金：浙江省科技厅重大科技攻关资助项目(2005C13026);浙江省科技厅基金资助项目(2004C33009)

摘　　要：采用RP-HPLC法,分别于SD大鼠灌胃给予鸡肝散提取物后0.17、0.5、1、2、4和6h,测定大鼠胃肠道组织中游离以及总的木犀草素的含量,以及血浆、心、肝、肺、肾中总的木犀草素的含量。结果表明,鸡肝散提取物中主要成分木犀草素苷在肠道中迅速水解成木犀草素而被吸收,在胃肠道组织中主要以苷元木犀草素形式存在。木犀草素经吸收后快速分布至各组织,其中代谢比较旺盛的肝、肾组织中木犀草素的含量高于心、肺组织中;肾、心、肺组织中的木犀草素含量均为1h时达最大,这与血药浓度达峰时间相似;而在肝组织中,药物分布迅速,0.17h已达高峰;在其他组织中因检测灵敏度问题而未被检出。建立的大鼠生物样本中木犀草素的含量测定方法具有灵敏、准确、专属性强等特点,可用于鸡肝散药效成分木犀草素的体内药动学研究。The study is aimed to establish a RP-HPLC method for determination of luteolin from the extracts of Elsholtzia blanda （EEB） in rats＇ biological specimen. A RP-HPLC method was established for determination of free and total luteolin in SD rats＇ plasma and gastrointestinal tract and total luteolin in SD rats＇ heart, liver, lung and kidney at 0.17, 0.5, 1, 2, 4 and 6 h after administration of EEB to 24 male SD rats （4 rats per one time spot）. Luteolin glycoside was hydrolyzed to aglycone luteolin in intestinal tract, and then luteolin was absorbed. The main form of luteolin existed in gastrointestinal tract after administration of EEB is aglycone. The content of luteolin in liver and kidney were higher than that in heart and lung. The content of luteolin in kidney, heart and lung were showed max at 1 h. Its peak time was similar to that in blood. However, in liver, the drug was distributed quickly, and showed max at 0.17 h. And because of the sensitivity of the method, luteolin was not be detected in other tissues. The method is sensitive, specific, accurate, and is appropriate for determination of luteolin in vivo.

关 键 词：高效液相色谱 鸡肝散 木犀草素 吸收 分布 

分 类 号：R969[医药卫生—药理学]