1,1-二氯乙烷微生物降解菌群的生理学鉴定(英文)  

Physiological characterization of a microbial consortium that reductively dechlorinates 1,1-dichloroethane to chloroethane

作　　者：杨锐[1] 孙宝林[1] 

机构地区：[1]合肥微尺度物质科学国家实验室,中国科学技术大学生命科学学院,安徽合肥230027

出　　处：《中国科学技术大学学报》2009年第1期83-89,共7页JUSTC

基　　金：Supported by National Natural Science Foundation of China(30470943)

摘　　要：从纽约哈得逊河河底淤泥样品中富集分离得到的微生物菌群,在厌氧条件下,可将1,1-二氯乙烷通过还原脱氯的呼吸代谢方式降解生成一氯乙烷.这个微生物菌群可以利用氢气、甲酸、乙酸、苯甲酸和延胡索酸作为电子供体进行还原脱氯生长,并能以丙酮酸发酵的呼吸方式生长,但丙酮酸不能用来作为电子供体进行还原脱氯.通过对其他氯代化合物是否能作为电子受体的实验,发现只有1,1-二氯乙烷能被脱氯.在培养基中加入亚硫酸盐可完全抑制二氯乙烷的还原脱氯,但硫酸盐、硝酸盐和延胡索酸盐的加入却不影响还原脱氯.在以氢气作为电子供体进行还原脱氯时,每摩尔氯原子的还原相应地使细胞干重增加(4.402±1.241)g.同时对富集分离的菌群进行16S rRNA基因序列分析发现,在脱氯生长方式下,该微生物菌群中Dehalobacter细菌占主导地位.An anaerobic microbial consortium reductively dechlorinating 1,1-dichloroethane （DCA） to chloroethane （CA） was enriched from the sediment of Hudson River, New York. The consortium used hydrogen, formate, acetate, benzoate and fumarate as electron donors for reductive dechlorination. Pyruvate fermentation occurred in the enrichment culture, although this compound was not used as an electron donor for dechlorination. Among other halogenated compounds tested, only DCA was reductively dechlorinated. Sulfite could completely inhibit DCA dechlorination, whereas sulfate, nitrate or fumarate had no effect. Growth by reductive dechlorination with hydrogen as electron donor was revealed by a cell yield of （4. 402±1. 241）g of cells （dry weight） per mole of chloride released. Sequence analysis of 16S rRNA gene amplified from a highly enriched culture with Hz as electron donor suggests that Dehalobacter species dominated the enrichment culture under dechlorination conditions.

关 键 词：1 1-二氯乙烷 还原脱氯 卤代呼吸 Dehalobacter 16S RRNA基因 

分 类 号：Q938.1[生物学—微生物学]