半枝莲多糖的纯化及对S_(180)荷瘤小鼠的抑瘤作用  

Purification and Determination of Polysaccharide from Scutellaria barbata D. Don and Tumor-Inhibitory Effect of Polysaccharide in Mice Bearing of Sarcoma-180

作　　者：张秀娟[1,2] 张丽娟[1,2] 张晶[3] 季宇彬[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学中心 [2]哈尔滨商业大学药物研究所博士后工作站抗肿瘤天然药物教育部工程研究中心 [3]哈尔滨商业大学药学院,哈尔滨150076

出　　处：《中国现代应用药学》2009年第1期6-8,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的将半枝莲粗多糖纯化后测定多糖含量,并比较粗多糖与精多糖的抑瘤率,为半枝莲化学及药理研究提供有益的参考。方法采用分光光度法、苯酚-硫酸显色法,在490 nm处测定其含量;并利用体内实验测定多糖的抑瘤效果。结果精多糖的多糖含量为54.12%,平均加样回收率为99.66%,粗多糖的抑瘤率为45.96%,精多糖的抑瘤率为22.14%。结论测定多糖含量的方法操作简便,准确可靠,精多糖的抑瘤效果没有粗多糖抑瘤效果明显。OBJECTIVE To detect the content of purifieatus polysaccharide from SPS, and compare the tumor-inhibitory of crude materials and purificated polysaecharide and provide some references for research on chemistry and pharmacology of polysaccharide from cutellaria barbara D. Don. METHODS UV-spectrophotometer and phenol-sulfuric method were used, the detection wavelength was 490 mn and tumor-inhibitory effect of polysaccharide was detected through intracorporeal test. RESULTS The percent of purificatus polysaccharide was 54.12% ,average recovery was 99.66% , the rate of inhibiting tumor of crude and purificatus polysaceharide were 45.96% and 22.14% respectively. CONCLUSION This method is simple, convenient, reliable and the anti-tumor activity of crude polysaccharide is better than that of purificatus polysaeeharide.

关 键 词：半枝莲 多糖 含量测定 硫酸苯酚法 抑瘤率 

分 类 号：R931.6[医药卫生—生药学] R963[医药卫生—药学]