谷氨酰胺对脓毒症小鼠的保护作用  

Protective effects of glutamine on septic mice

作　　者：袁媛[1] 王学敏[1] 薛瑛[1] 江伟[1] 

机构地区：[1]上海交通大学附属第六人民医院麻醉科,200233

出　　处：《上海医学》2009年第1期58-61,共4页Shanghai Medical Journal

基　　金：上海市科学技术委员会科研计划项目(054119516)

摘　　要：目的探讨谷氨酰胺对脓毒症小鼠炎性反应及生存率的影响。方法昆明小鼠45只,随机分为假手术组(CON)、手术组(CLP)、谷氨酰胺组(GLN),每组15只。采用盲肠结扎穿孔法(CLP)制备脓毒症模型,术后即刻尾静脉注射谷氨酰胺0.75g/kg(GLN组)或等体积0.9%氯化钠溶液(CON和CLP组)。6h后每组各取5只,收集血清,腹腔灌洗分离巨噬细胞,采用酶联免疫吸附试验(ELISA)测定血清和巨噬细胞中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、IL-10水平;另外每组各取10只观察术后5d生存率。结果CLP和GLN组血清中TNF-α、IL-6、IL-10水平均显著高于CON组(P值分别<0.05、0.01);GLN组血清TNF-α水平显著低于CLP组(P<0.05),两组间血清IL-6、IL-10的差异均无统计学意义(P值均>0.05)。GLN组巨噬细胞中TNF-α、IL-6水平均显著低于CLP组(P值分别<0.01、0.05),3组间IL-10水平的差异无统计学意义(P>0.05)。GLN组小鼠的存活时间显著长于CLP组(P<0.05)。结论谷氨酰胺可以抑制脓毒症小鼠的炎性反应,降低其病死率。Objective To investigate the protective effects of glutamine （Gin） on septic mice. Methods Forty-five Kunming mice were randomly divided into three groups （n = 15 each）： sham-operated group （CON）, sepsis model group （CLP）, and Gin-treated group （GLN）. Sepsis was induced by cecal ligation and puncture. Either glutamine （0.75 g/kg） or saline was administered immediately after surgery via single tail-vein injection and the survival rate was observed. Macrophages were harvested by peritoneal lavage at 6 h. Both intracellular and serum cytokines were assayed via ELISA. Results Serum IL-6 and IL-10 levels were similar between two groups （all P〉0.05） ; the serum level of TNF-α in GLN group was significantly lower than that in CLP group （P〈0.05）. Intracellular TNF-α and IL-6 levels were also significantly lower in GLN group compared with CLP group （ P〈0.01, 0.05）, but the intracellular IL-10 level was similar among the 3 groups （P〉0.05）. Survival rate at 5 days post treatment was significantly higher in GLN group （30%） than that in the GLP group （10%, P〈0.05）. Conclusion Gin treatment exerts an anti-inflammatory effect in vivo during sepsis. The anti-inflammatory effect of Gin is also associated with improvement of this septic mouse survival.

关 键 词：谷氨酰胺 脓毒症 炎性因子 巨噬细胞 

分 类 号：R459.7[医药卫生—急诊医学]