超声合成Fe3O4@SiO2复合纳米磁性粒子用于质粒DNA的提纯  

Ultrasonic Synthesis of Fe_3O_4@SiO_2 Nano-composite Magnetic Particles for Plasmid DNA Purification

作　　者：王显祥[1,2] 王森[1] 杨婉身[1] 单志[1] 

机构地区：[1]四川农业大学生命科学与理学院,雅安市625014 [2]四川大学化学学院,成都610041

出　　处：《化学学报》2009年第1期54-58,共5页Acta Chimica Sinica

基　　金：四川省教育厅自然科学科研重点项目(No.2005A033);四川农业大学引进人才基金(No.007202)

摘　　要：用超声合成了Fe3O4@SiO2复合纳米磁性粒子,并用于质粒DNA的提取.用透射电镜(TEM)、红外光谱(FT-IR)、震动样品磁场计(VSM)、X光电子能谱仪(XPS)等方法对合成的复合磁性粒子的表面形貌、结构、磁性质等进行了表征,合成的复合磁微粒粒径分布均匀,在15～20nm,磁响应性好.用该复合磁微粒提取DNA的纯度能达到A260/A280=1.8±0.1,琼脂糖电泳证明质粒DNA结构基本没有被破坏,主要为超螺旋结构,能满足PCR等后续分子生物学操作的要求.Fe3O4@ SiO2 core-shell nanoparticles for plasmid DNA purification were prepared by ultrasound and investigated by transmission electron microscopy （TEM）, infrared spectrum （FT-IR）, sample vibrating magnetometry （VSM） and X photoelectron energy spectrum （XPS） in order to elucidate the morphology, magnetic and other properties of the nano-composites. The results showed that they possessed uniform distribution of particle size, which was about 20 nm, magnetic responsiveness, and even distribution, and could be applied to the magnetic composite nanoparticles to extract plasmid DNA. The A260/A280 ratios of DNA samples were 1.8±0.1. Through agarose gel electrophoresis, the DNA structure was not damaged and was of superhelix. The plasmid DNA could be used for PCR and other molecular biology operation.

关 键 词：复合纳米磁性粒子 超声 Fe3O4@SiO2 DNA 提取 

分 类 号：TB383.1[一般工业技术—材料科学与工程]