激发发射荧光矩阵二阶校正方法直接测定人体血浆样中多沙唑嗪含量  

Direct determination of doxazosin in human plasma samples using 3-D fluorescence matrix coupled with second-order calibration methods

作　　者：万瑶[1] 吴海龙[1] 韩清娟[1] 李元娜[1] 张燕[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《计算机与应用化学》2009年第1期5-8,共4页Computers and Applied Chemistry

基　　金：国家自然科学基金资助项目(20775025，20435010);国家973预研课题(2007CB216404)．

摘　　要：多沙唑嗪是一种α-1受体阻滞剂,有选择性地作用于α-1受体,可改善良性前列腺增生(BPH)症状,又可降低血压。化学计量学二阶校正算法具有独特的"二阶优势",能够在未知干扰组分共存下,直接定量分析体系中感兴趣的组分。本文基于多沙唑嗪稳定的荧光性质,利用激发发射荧光矩阵(EEMs)结合二阶校正算法,成功地直接测定血浆样中多沙唑嗪。血浆样品不需做前期处理。它快速简便,比其它分析方法,既节约试剂又节约时间,是一种对复杂体系感兴趣组分进行直接定量分析的好工具。Doxazosin is a selective inhibitor of the alphal subtype of alpha adrenergic receptors. It was used in men to treat the symptom of benign prostatic hyperplasia（BPH） with hypertension. Second-order calibration with its second-order advantage can be applied for a direct determination of interested components, even in the presence of unknown interferences. Benefited from doxazosin＇s stable fluorescence character, this paper proposed a new and effective approach for direct determination of doxazosin in human plasma samples by the incorporation of excitation-emission matrix （EEMs） fluorescence and second-order calibration. This strategy that allowed omitting the pretreatment of plasma samples helps us save time and reagents, and was convenient. Second-order calibration method was promising tool for determination of interested analytes in complex mixture.

关 键 词：多沙唑嗪 血浆样 激发发射荧光矩阵 二阶校正 

分 类 号：O656[理学—分析化学] TP319[理学—化学]