猪离体支气管温缺血研究  

Isolated bronchi from non-heart-beating pig

作　　者：杨洋[1] 王建军[1] 赵松[2] 廖秋明[3] 

机构地区：[1]华中科技大学同济医学院附属协和医院胸外科,武汉430022 [2]郑州大学第一附属医院胸外科 [3]瑞典隆德大学医院心胸外科

出　　处：《中华实验外科杂志》2009年第2期219-221,共3页Chinese Journal of Experimental Surgery

摘　　要：目的通过测定和比较有心跳供体（HBD）和温缺血1h后的无心跳供体（NHBD－1h）肺的细支气管平滑肌舒缩功能和上皮依赖性舒张（EpiDR）能力，探讨NHBD－1h肺用于肺移植的可行性。方法16头瑞典家猪随机分为HBD组和NHBD－1h组，从它们身上获取内径约1．5mm的支气管段，截至1．5mm长的无分支的支气管环，置入器官浴槽装置，用乙酰胆碱、花生四烯酸钠等药物测量两组支气管环对它们的反应，进而对两组平滑肌舒缩功能和EpiDR能力进行对比。结果HBD组和NHBD－1h组对比，由乙酰胆碱所诱导的支气管平滑肌收缩能力间的差异（5．18±0．07比5．10±0．11）无统计学意义（P〉0．05）；两组气管平滑肌均可对盐酸罂粟碱产生完全的舒张；在排除HBD和NHBD－1h组自发性舒张的干扰后（HBD－B：5．18±0．10比5．20±0．11，P〉0．05；NHBD－1h—B：5．10±0．06比5．11±0．07，P〉0．05），其EpiDR能力的差异（1．26±0．05比1．23±O．07）亦无统计学意义（P〉0．05）。结论温缺血1h后，无论支气管平滑肌的收缩还是舒张功能均保存完好，肺支气管的上皮依赖性调节和非缺血肺支气管的差异无统计学意义。Objective By measuring and comparing the relaxant and contractile abilities of lung bronchial smooth muscle and the epithelial dependent relaxation （EpiDR） ability between heart-beating donor （HBD） and non-heart-beating-1 h donor （NHBD-1 h） ,to explore the feasibility of the NHBD-1 h lung to be used for lung transplantation. Methods Sixteen Swedish pigs were divided into two groups randomly：HBD group and NHBD-1 h group. Several bronchial tings were harvested from these pigs with inner diameters of all about 1.5 mm and about 1.5 mm long without branches, and then placed into the organ bath device. The reactions of the tings to some certain drugs [ acetylcholine （ Ach）, arachidonie acid natriurn, etc. ] were tested, and the relaxant and contractile abilities of lung bronchial smooth muscle and EpiDR ability analyzed. Results Between HBD group and NHBD-1 h group, there was no significant difference in contraction of bronchial smooth muscle caused by acetylcholine （5.18 ± 0.07 vs 5.10 ± 0.11 ） （ P 〉 O. 05 ） ; bronchial smooth muscles in both groups could were completely relaxed by papaverin hydroehloride; after removing the spontaneous relaxation interference in HBD and NHBD-1 h groups （HBD-B：5.18±0.10 vs 5.20±0. 11,P〉0.05;NHBD-I h-B：5.10 ±0.06 vs 5.11±0.07,P 〉0.05）, there was no significant differences in average EpiDR ability （1.26 ±0.05 vs 1. 23 ± O. 07 ） between two groups （P 〉 0.05） also. Conclusion After warm ischemia for 1 h, the relaxant and contractile abilities of lung bronchial smooth muscle keep intact, and the epithelial dependent adjustment of lung bronchi shows no significant difference between HBD and NHBD-1h.

关 键 词：支气管 缺血 平滑肌 

分 类 号：R285.5[医药卫生—中药学]