可溶性白细胞分化抗原14对严重烫伤内毒素血症小鼠血清促炎和抗炎细胞因子的影响  

Effects of Soluble Cluster of Differentiation 14 on Serum Levels of Pro-and Anti-inflammatory Cytokines in Mice with Serious Scald Endotoxemia

作　　者：于永洲[1] 王占科[1] 潘小清[1] 

机构地区：[1]解放军第94医院,南昌市330002

出　　处：《中国药房》2009年第4期250-251,共2页China Pharmacy

基　　金：南京军区医药卫生"十一五"重大课题(06Z25)

摘　　要：目的:观察可溶性白细胞分化抗原14(sCD14)对严重烫伤内毒素血症模型小鼠血清促炎和抗炎细胞因子的影响。方法:60只健康昆明种小鼠随机均分为sCD14(5mg·kg-1)治疗组、治疗对照组(生理盐水)和正常对照组(生理盐水)。前2组建立30%的体表面积Ⅲ度烫伤模型后每隔12h尾静脉注射相应药物,连续3d。第4天断头处死收集血样检测血清促炎细胞因子(TNFα和IL-6)和抗炎细胞因子(IL-4和IL-10)水平变化。结果:sCD14治疗组血清TNFα和IL-6水平均明显低于治疗对照组(P<0.01),但仍高于正常对照组(P<0.01);sCD14治疗组血清IL-4和IL-10水平与治疗对照组比较无显著变化。结论:sCD14可能通过抑制严重烫伤内毒素血症小鼠血清促炎细胞因子分泌而降低炎症反应。OBJECTIVE： To study the effects of soluble cluster differentiation 14 （sCD14） on serum pro- and anti- inflammatory cytokines in mice with serious scald endotoxemia. METHODS： A total of 60 healthy Kunming mice were randomly assigned to sCD14 （5 mg·kg^-1） group, treatment control group （normal saline） and normal control group （normal saline） . The first two groups were established into scald model （scald of 30% body surface area, Ⅲ degree scald） followed by intravenous injection of the corresponding drugs in every 12 h via vena caudalis for 3 consecutive days. On day 4, the model mice were sacrificed to obtain the serum sample for the detection of levels of proinflammatory cytokines （TNFα, IL- 6） and anti - inflammatory cytokines （IL- 4 and IL- 10） . RESULTS： In sCD14 treatment group, serum levels of TNFα, IL -6 were significantly lower than in treatment control group （P 〈 0.01）, but were still higher than in normal control group （P 〈 0.01）. No significant differences were noted between sCD14 treatment group and the treatment control group in serum levels of IL - 4 and IL 10. CONCLUSION ： The anti - inflammatory action of sCD14 on mice with endotoxemia induced by serious scald might be achieved by down- regulating serum levels of proinflammatory cytokines.

关 键 词：可溶性白细胞分化抗原14 烫伤 小鼠 内毒素 促炎细胞因子 抗炎细胞因子 

分 类 号：R965[医药卫生—药理学]