高效液相色谱法测定调肝灵片中大黄素和大黄酚的含量  

Determination of emodin and chrysophanol in tiaoganling pills by HPLC

作　　者：刘玉斌[1] 

机构地区：[1]黑龙江中医药大学附属第一医院,哈尔滨150040

出　　处：《中国实用医药》2009年第3期20-21,共2页China Practical Medicine

摘　　要：目的建立以高效液相色谱法测定调肝灵片中大黄素、大黄酚含量的方法。方法色谱柱:以十八烷基硅烷键合硅胶为填充剂,流动相:甲醇-0.1%磷酸溶液(85:15)为流动相;流速:1.0ml/min;检测波长:254nm;柱温:30℃,进样量10μl。结果大黄素和大黄酚分别在0.0056～0.028μg之间和0.0112～0.056μg(r=0.9998)之间成线性关系。大黄素、大黄酚总量平均回收率为100.13%(RSD=2.53%,6例)。结论本方法简便、准确、重现性好。Objective To establish a quality standard of Tiaoganling pills. Methods Hypersil C18 was used with methanol-0. 1% phosphoric acid as mobile phase （85：15）. The flow rate was 1.0 mL/min, detective wavelength was 254 nm,column temperature was 30℃ and injection valume was 10μl. Results Emodin and chrysophanol showed a good linear relationship in the range of 0. 005 6 -0. 028 μg and 0. 011 2- 0. 056 μg（ r =0. 999 8）. The average recovery was 100. 13% （RSD =2. 53% ,6）. Conclusion The methods is simple,accurate and reproducible.

关 键 词：调肝灵片 高效液相色谱法 大黄素 大黄酚 含量测定 色谱法 

分 类 号：R286.0[医药卫生—中药学] R512.62[医药卫生—中医学]