电化学免疫传感器研制过程中电极的选择与改进  

Selection and improvement of electrodes used in developing electrochemical immunosensor

作　　者：赵广英 马超 

机构地区：[1]浙江工商大学食品与生物工程学院,浙江省食品安全重点实验室,杭州310035

出　　处：《食品科技》2009年第1期101-107,共7页Food Science and Technology

基　　金："十一五"国家863计划海洋技术领域重点项目(2007AA091806);国家自然科学基金资助项目(30571623);浙江省重中之重项目(Z05-178);浙江省食品质量安全重点实验室开放项目(Z04-179)

摘　　要：支持电极是影响免疫传感器发展的关键技术问题之一。通过近5年免疫传感器的研究,对支持电极进行了系列选择和研究改进工作。在研究中选用过3种固体单电极,单通道丝网印刷碳电极,改进制备的四通道、五通道和八通道丝网印刷碳电极。采用激光扫描共聚焦显微镜(LSCM)进行形态学表征,通过循环伏安法(CV)进行电化学表征,评估电极的性能,初步选定四通道丝网印刷碳电极(4-SPCE)为支持电极。用物理吸附法制备辣根过氧化物酶(HRP)酶电极,以检验选定的4-SPCE的应用性能。结果显示,改进制备的4-SPCE的应用性能最好,因此选用该4-SPCE用作电化学免疫传感器研究中的支持电极。Supporting electrode was one of the key technologies in the development of immunosensor. Through the research on immunosensors of the last five years, supporting electrode had been chosen and improved; three kinds of solid electrodes, single-channel screen-printed carbon electrode （SPCE）, improved four-channel, five-channel and eight-channel SPCE, were selected. The electrodes were characterized by laser scanning confocal microscope （LSCM） and electrochemical method based on cyclic voltammetry（CV）. Four-channel screen-printed carbon electrode （4-SPCE） was selected as preliminary supporting electrode. Horseradish peroxidase （HRP） electrode was prepared by physical adsorption. The enzyme electrode was used to test the performance of the 4-SPCE, the result of experiment indicated that improved 4-SPCE had the most excellent application performance. Therefore, the 4-SPCE was used as supporting electrode in developing electrochemical immunosensor.

关 键 词：电化学免疫传感器 支持电极 固体电极 四通道丝网印刷碳电极 酶电极 辣根过氧 化物酶 

分 类 号：TS212.3[轻工技术与工程—粮食、油脂及植物蛋白工程]