一株具有抗肿瘤活性的海洋放线菌的分离和鉴定  

Isolation and Identification of One Marine Actinomycete Strain Exhibiting Antitumor Activity

作　　者：袁献温[1] 杨瑞丽[1] 

机构地区：[1]东南大学医学院病原生物学和免疫学系,江苏南京210009

出　　处：《微生物学通报》2009年第1期78-83,共6页Microbiology China

基　　金：江苏省卫生厅课题(No.H200758)

摘　　要：海洋放线菌ACMA006的发酵产物具有很强的抗肿瘤活性和较好的抑菌效果,但对人常细胞毒性较弱。该菌株在大多数培养基上生长良好,产生可溶性色素;基质菌丝在培养早期现横隔,随后发生断裂;最适生长温度为28°C。基于16SrDNA序列的系统发育分析表明,菌ACMA006属于链霉菌属(Streptomyces)的成员,与该属的合格发表种卡伍尔链霉菌华盛顿亚(S.cavourensis subsp.washingtonensis)的16SrDNA序列相似性最高,同源性达100%,但在部分态特征和理化特性上存在较大差异。初步确定ACMA006属于卡伍尔链霉菌华盛顿亚种的一个洋变种。The fermentation broth of one actinomycete strain ACMA006 strongly inhibited growth of many tumor cells and some microorganisms, but its cytotoxicity to human normal cells were weak. Strain ACMA006 grow well on most tested media, producing exuberant vegetative hyphae and aerial hyphae. Its optimization temperature is 28℃. Phylogenetic analysis based on 16S rDNA sequence showed that strain ACMA006 was closely related to one of the genus Streptomycetes （S.cavourensis subsp, washingtonensis） with 16S rDNA sequence similarity values of 100%, but had many differences in other features including its morphology, physiological and biochemical characteristics. The preliminary study supported the view that the strain ACMA006 represented a new strain of the S.cavourensis subsp, wash- ingtonensis.

关 键 词：抗肿瘤活性 海洋放线菌 16S RDNA 系统发育分析 

分 类 号：Q93[生物学—微生物学]