Degradation of o-chloronitrobenzene as the sole carbon and nitrogen sources by Pseudomonas putida OCNB-1  

作　　者：WU Haizhen WEI Chaohai WANG Yaqin HE Qincong LIANG Shizhong 

机构地区：[1]College of Bioscience and Engineering, South China University of Technology, Guangzhou 510640, China. [2]College of Environmental Science and Engineering, South China University of Technology, Guangzhou 510640, China

出　　处：《Journal of Environmental Sciences》2009年第1期89-95,共7页环境科学学报（英文版）

基　　金：supported by the National Natural Sci- ence Foundation of China (No. 50278036);the National Hi-Tech Research and Development Program (863) of China (No. 2006AA06Z378)

摘　　要：A bacterial strain that utilized o-chloronitrobenzene （o-CNB） as the sole carbon, nitrogen and energy sources was isolated from an activated sludge collected from an industrial waste treatment plant. It was identified as Pseudomonas putida based on its morphology, physiological, and biochemical characteristics with an automatic biometrical system and the 16S rRNA sequence analysis. Microcosm study showed that the biodegradation of o-CNB was optimized at culture medium pH 8.0 and 32℃. At these conditions, the strain degraded 85% of o-CNB at a starting concentration of 1.1 mmol/L in 42 h. o-Chloroaniline was identified as the major metabolite with high performance liquid chromatography （HPLC） and gas chromatography-mass spectrometry （GC-MS）. The study showed that o-CNB degradation by Pseudomonas putida OCNB-1 was initiated by aniline dioxyenase, nitrobenzene reductase and catechol-l,2- dioxygenase.A bacterial strain that utilized o-chloronitrobenzene （o-CNB） as the sole carbon, nitrogen and energy sources was isolated from an activated sludge collected from an industrial waste treatment plant. It was identified as Pseudomonas putida based on its morphology, physiological, and biochemical characteristics with an automatic biometrical system and the 16S rRNA sequence analysis. Microcosm study showed that the biodegradation of o-CNB was optimized at culture medium pH 8.0 and 32℃. At these conditions, the strain degraded 85% of o-CNB at a starting concentration of 1.1 mmol/L in 42 h. o-Chloroaniline was identified as the major metabolite with high performance liquid chromatography （HPLC） and gas chromatography-mass spectrometry （GC-MS）. The study showed that o-CNB degradation by Pseudomonas putida OCNB-1 was initiated by aniline dioxyenase, nitrobenzene reductase and catechol-l,2- dioxygenase.

关 键 词：O-CHLORONITROBENZENE Pseudomonas putida CHARACTERIZATION DEGRADATION 

分 类 号：X7[环境科学与工程—环境工程]