生防真菌遗传转化方法研究进展  

Research progress of genetic transformation methods of biological control fungi

作　　者：刘昌燕[1] 赵培静[1] 汪军[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《中国植保导刊》2009年第1期13-16,共4页China Plant Protection

基　　金：国家科技基础条件平台工作(2005DKA21201);中央级公益性科研院所基本科研业务费专项(2007HZS1J007)

摘　　要：生防真菌是一类非常重要的环境微生物,在防治有害生物中具有广阔的发展潜力。近年来,都采用菌株改良的途径来提高生防真菌的毒力。其中基因工程技术被认为是最有效的菌株改良途径,成为研究生防真菌分子生物学的新工具。为此,对生防真菌的遗传转化方法进行了综述。主要包括原生质体转化、电激转化、基因枪法、限制性内切酶介导转化(REMI)法、根癌农杆菌介导转化法,并对其应用前景进行了展望。Biological control fungi was a kind of important environment microorganism that had much potential in the field of pest control. In recent yeats, it was by strains promotion to improve the toxicity of biological control fungi. As the most effective method to promote strains, genetic engineering had been a new tool to study molecular biology of biological con- trol fungi. Genetic transformation methods of biological control fungi, including protoplast transformation, electrization transformation, methods of particle gun, restriction enzyme-mediated integration, and transformation mediated by agrobacterium tumefaciens, and the application prospects were reviewed in this paper.

关 键 词：生防真菌 遗传转化方法 展望 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治] Q319.2[农业科学—植物保护]