几丁聚糖纳米混悬剂研制及其表征  

Preparation and Character of Chitosan Nanoparticles

作　　者：蒋玉燕[1] 毕忆群[1] 房国坚[1] 蒋建国[1] 

机构地区：[1]浙江省医学科学院生物工程研究所,杭州310013

出　　处：《浙江省医学科学院学报》2008年第1期6-8,5,共4页

基　　金：浙江省科技厅重点计划项目资助（2006F12037）;浙江省科技厅重大计划项目资助（2005F1101）

摘　　要：目的运用理化手段，进行几丁聚糖纳米（nanoparticles）混悬剂制备及其表征。方法2006年07月-2007年06月运用化学酸解、Co-60γ射线辐照、高压、超声波及稳定剂等综合理化手段制备几丁聚糖纳米粒子。应用透射电镜（TEM）和激光动态光散射仪（Zetasizer3000HS）进行几丁聚糖纳米粒子尺寸、形貌及分布的表征。结果制备的几丁聚糖纳米粒子为类圆球形；平均粒径25．3nm，占总粒子数99．1％，粒径分布图形较窄，表明颗粒大小均匀。结论运用以Co-60γ射线辐照为主要手段进行几丁聚糖纳米混悬剂的制备，是一种简便、快速、无污染、重复性好的方法，具备可行性。Objective The Chitosan nanoparticles were preparated by physical and chemical methods, then to character the morphology, size and distribution. Methods The experiment was performed in the institute of bioengineering,Zhejiang Academic of Medical Sciences from Jul.2006 to Jun. 2007. At first, chitosan nanoparticle was produced by chemical acid reaction, Co-60γ radiation, autoclave and ultrasonic. The chitosan nanoparticle morphology.size and distribution were charactered by transmission electron microscope（TEM） and laser particle sizer（Zetasizer3000HS）. Results Chitosan nanoparticles were sphere, with average diameter 25.3nm.Size distribution percentum was 99.1% and distribution was consistent. Conclusion Chitosan nanoparticle was produced by Co-60γ, radiation which was simple and celerity.

关 键 词：几丁聚糖 纳米粒子 制备 表征 

分 类 号：R944.9[医药卫生—药剂学] R151.2[医药卫生—药学]