pres基因打靶载体同源臂的PCR引物设计  

Design of PCR primer for targeting vector homology arm of pres gene

作　　者：徐灵活[1] 章建程[1] 丁猛[1] 王晓花[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《海军医学杂志》2008年第4期289-291,共3页Journal of Navy Medicine

基　　金：海军医学研究所科研基金(06HY31)

摘　　要：目的:设计pres基因打靶载体同源臂的PCR引物。方法:在对pres基因结构和功能进行分析之后,选取目标扩增区域进行针对性的酶切位点分析,基于引物设计原则,在符合酶切位点要求的区域内设计同源臂PCR引物。结果:用于构建打靶载体同源长短臂的引物设计符合研究要求。结论:在考虑引物设计原则和酶切位点分析的基础上,可成功设计打靶载体同源臂PCR引物。Objective： To design PCR primer for targeting vector homology arm of pres gene. Methods： After an analysis on structure and function of pres gene, an objective amplification zone was selected to make an analysis on enzyme cutting sites. Then, on account of the principle of primer design, the homology arm PCR primer was designed on the zone consistented with the requirements of enzyme cutting sites. Results： The primer designed for targeting vector homology arm could meet this research requirement. Conclusions： Based on the principle of primer design and the analysis of enzyme cutting sites, PCR primer for targeting vector homology arm can be designed successfully.

关 键 词：pres基因 PCR引物 引物设计 

分 类 号：R393-33[医药卫生—基础医学]