蜂胶、杨树叶及杨树芽中水杨苷和芦丁含量比较  

Comparative study:Salicin and Rutin Contents in Propolis,and Poplar Leaves and Buds

作　　者：南垚[1] 周立东[1] 

机构地区：[1]中国医学科学院北京协和医科大学药用植物研究所,北京100193

出　　处：《世界科学技术-中医药现代化》2008年第6期59-61,共3页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

摘　　要：目的:建立用高效液相同时测定样品中水杨苷和芦丁含量的方法。方法:样品经过50%乙醇超声提取,定容,以甲醇:1.5%磷酸为流动相,以HPLC测定其中水杨苷和芦丁的含量。结果:水杨苷线性范围1.23～12.3μg,r=0.9993,平均回收率101.1%,RSD1.7%;芦丁线性范围1～10μg,r=0.9998,平均回收率l02.2%,RSD1.8%。结论:本法简单,分离度好,重复性良好,可用于测定样品中水杨苷和芦丁含量。蜂胶中不含芦丁等苷类物质,我国蜂胶行业标准值得商榷。Authors developed an HPLC method to determine the contents of Salicin and Rutin in Propolis. The separation of the two components was achieved on a reversed-phase Kromasil C18 column with MeOH-H20 （1.5%H3PO4）gradient elution at a flow rate of 1.0 mL.min-1. Results show that the calibration curves were linear in the range of 1.23- 12.3μg for Salicin （r=0.9993）, and 1-10μg for Rutin （r=0.9996）, with an averaged recovery at 101.1% and 102.2% respectively. It is believed that Salicin and Rutin are not existed in Propolis. The proposed method is simple, accurate, and reliable, desirable for determining Salicin and Rutin in poplar leaves and buds.

关 键 词：蜂胶 水杨苷 芦丁 HPLC 含量测定 

分 类 号：R284.2[医药卫生—中药学] R284.1[医药卫生—中医学]