通痹灵对类风湿关节炎滑膜细胞血管内皮生长因子mRNA的影响  

Effect of Tongbiling on VEGF mRNA Expression in Rheumatoid Arthritis Synovial Cell

作　　者：黄清春[1] 储永良[1] 接力刚[1] 沈鹰[1] 陈纪藩[2] 陈光星[2] 王捷[1] 

机构地区：[1]中国人民解放军广州军区广州总医院,广东广州510010 [2]广州中医药大学第一附属医院,广东广州510000

出　　处：《中国中医药信息杂志》2009年第2期34-35,40,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：中国博士后科学基金(20070420140);广东省自然科学基金(20020656);广东省中医药管理局科技项目(1040139)

摘　　要：目的观察通痹灵对体外培养的类风湿关节炎(RA)滑膜细胞血管内皮生长因子(VEGF)mRNA表达的影响,探讨其可能的作用机制。方法行关节腔镜手术取RA患者膝关节滑膜组织消化分离滑膜细胞进行分组培养。空白对照组:含生理盐水+10%胎牛血清的DMEM培养;通痹灵低剂量组:50mg/L通痹灵+10%胎牛血清的DMEM培养;通痹灵高剂量组:200mg/L通痹灵+10%胎牛血清的DMEM培养。提取总RNA,逆转录-聚合酶链反应(RT-PCR)法检测各组VEGFmRNA的表达。结果通痹灵高、低剂量组VEGFmRNA表达较对照组明显降低(P<0.05)。结论通痹灵可以下调RA患者滑膜细胞VEGFmRNA表达水平,从而可以通过抑制VEGF表达减轻滑膜血管翳增殖。Objective To study effect of Tongbiling （TBL） on VEGF mRNA expression in RA synovlcytes cultured in vitro and explore its mechanism. Method Synovial cells from the knee joint in patient with RA were digested, divided and separately cultured after arthroscopy. Blank controls： DMEM culture containing 10% FBS in saline; Low-dose admission group of TBL： DMEM culture containing 10% FBS in 50 mg/L TBL; High-dose admission of TBL： DMEM culture containing 10% FBS in 200 mg/L TBL. RNA were distilled. Expression of VEGF mRNA were detected with RT-PCR. Result Compared with control group, expression of VEGF mRNA in low and high-dose admission groups of TBL both decreased （P〈0.05）. Conclusion Expression of VEGF mRNA can be decreased by TBL, so the proliferation of the synovial pannus can be alleviated by inhibition of VEGF expression.

关 键 词：通痹灵 类风湿关节炎 滑膜细胞 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]