水稻苗期响应光强日变化的分子行为生态初探  

Primarily study on molecular behavior ecology of rice seedling in response to daily change in sunlight intensity

作　　者：林文雄[1] 余高镜[1] 熊君[1] 陈婷[1] 

机构地区：[1]福建农林大学农业生态研究所,福州350002

出　　处：《中国生态农业学报》2009年第1期115-119,共5页Chinese Journal of Eco-Agriculture

基　　金：国家自然科学基金项目(30571103);教育部生态学博士点基金项目(20050389004)资助

摘　　要：本研究以常规优质稻"佳辐占"为材料,应用差异蛋白组学的方法研究福建省光照最强月份(7月份)水稻秧苗功能叶响应光强日变化的分子机制。结果表明:共有7个蛋白质发生表达丰度的日变化,经质谱分析与功能鉴定,预测了6个蛋白质,分别是叶绿体光合系统Ⅰ反应中心亚基Ⅱ-类蛋白(蛋白点1)、核苷单磷酸激酶b(蛋白点2)、含Lon保守区的蛋白酶类蛋白(蛋白点3)、推定的还原酶(蛋白点4)、分子伴侣(蛋白点5)和倍半萜环化酶1(蛋白点6),其中,除蛋白点2、3在午间表达丰度增强之外,其余均下降,揭示了水稻功能叶蛋白质响应光强日变化的分子行为生态学特性。Commonly cultivated high quality elite rice cultivar ＂Jiafuzhan＂ was used to study molecular behavior ecological mechanisms in functional leaves of rice seedlings through investigation on their response to daily changes in sunlight intensity （from 6：00 to 18：00）. The results reveal 7 proteins with regular patterns of daily change in expression abundance and 6 of them are successfully identified and predicted. The proteins are chloroplast photosystem Ⅰ reaction center subunit Ⅱ - proteinoid （spot 1 protein）, UMP/CMP kinase b （spot 2 protein）, OSJNBa0027P08.12 protein （spot 3 protein）, putative reduetase （spot 4 protein）, putative chaperonin （spot 5 protein） and putative sesquiterpene cyclase 1 （spot 6 protein）. Expression abundance of all identified proteins drop at mid-day （12：00 ~ 14：00）, except for spots 2 and 3 proteins. This implies that the molecular behavior ecological properties are related to deferentially expressed protein abundance in functional rice leaves in response to changes in daylight.

关 键 词：水稻 光抑制 光强日变化 差异蛋白组学 分子行为生态特性 

分 类 号：Q948[生物学—植物学] S312[农业科学—作物栽培与耕作技术]