人参皂甙Rg-1对大鼠牙周组织中白介素6、骨钙素水平的影响  

Effects of ginsenoside Rg-1 on the expressions of interleukin-6,bone gla protein in periodontal tissues in periodontitis rats

作　　者：杨倩[1] 余占海[1] 杜建东[1] 张国英[1] 何福德[1] 

机构地区：[1]甘肃兰州大学口腔医学院,730000

出　　处：《实用口腔医学杂志》2009年第1期22-25,共4页Journal of Practical Stomatology

基　　金：甘肃省科技攻关基金项目(编号:2GS064-A43-020-34);兰州市攻关基金项目(编号:06-1-01)

摘　　要：目的:检测人参皂甙对牙周炎大鼠牙周组织中白介素6(interleukin-6,IL-6)及骨钙素(bone gla protein,BGP)的表达的影响并探讨其治疗牙周炎的机制。方法:选用100只体重160～200 g Wistar大白鼠,随机分成10组,每组10只。A组:正常对照组,0周时处死。B-J组建立牙周炎大鼠模型。B-E组:人参皂甙Rg-1治疗组,第1、2、3、4周处死。F-J组:牙周炎对照组,第0、1、2、3、4周处死。取大鼠上颌磨牙牙周组织标本作切片,光镜观察,免疫组化方法对IL-6、BGP进行染色,测量灰度值,进行统计学分析。结果:F组BGP水平明显低于A组,IL-6水平明显高于A组(P<0.05);C-E组BGP的水平明显高于H-J组,D-E组IL-6的水平明显低于I-J组(P<0.05)。结论:人参皂甙Rg-1可以抑制牙周炎大鼠牙周组织中IL-6的水平,同时升高BGP的水平,具有治疗牙周炎并改善全身状况的作用。Objective：To determine the effects of ginsenoside Rgl on the expression levels of bone gla protein （BGP） and cytokine interleukin-6 （IL-6） in the periodontium of experimental periodontitis rats. Methods：One hundred 160 - 200 g Wistar rats were randomly divied into ten groups. Group A used as control were killed at the beginning of the experiment. Group B-J were experimental periodontitis models. Group B-E received the therapy of ginsenoside Rgl were killed at 1st, 2nd, 3rd and 4th weeks. Group F-J received no treatment as periodontitis control were killed at 0, 1st, 2nd, 3rd and 4th weeks. The expression levels of BGP and IL-6 in the periodontium were measured by immuno- histochemistry. Results ：The expression levels of BGP in the group F were significantly lower than that in the group A, the expression levels of IL-6 in the group F were much higher than that in the group A（ P 〈 0.05 ）. The expression levels of BGP in the group C-E were significantly higher than that in the group H-J. The expression levels of IL-6 in the group D- E were much lower than that in the group I- J （ P 〈 0.05 ）. Conclusion ： The ginsenoside Rgl enhances the ex- pression levels of BGP and lowered the expression levels of IL-6 in the periodontium of experimental periodontitis models, it can treat periodontitis and improve the general physical conditions.

关 键 词：人参皂甙Rg-1 牙周炎模型 免疫组织化学 白介素6 骨钙素 

分 类 号：R285.5[医药卫生—中药学]