细胞凋亡在三氯乙烯致皮肤刺激性损伤中的作用  

Role of apoptosis in trichloroethylene induced irritant injury in the skin

作　　者：于均峰[1] 朱启星[1] 

机构地区：[1]安徽医科大学公共卫生学院职业卫生和环境卫生学系,合肥230032

出　　处：《中华劳动卫生职业病杂志》2009年第1期16-20,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金项目（30471469、30671787）;安徽医科大学校基金（2006kj15）

摘　　要：目的研究细胞凋亡在三氯乙烯（TCE）引起BALB／c裸鼠皮肤损伤小的作用并探讨其机制。方法将30只BALB/c裸鼠随机分为溶剂对照组、10％的TCE剂量组、20％TCE组、40％TCE组、80％TCE组、100％TCE组，应用TUNEL法检测TCE染毒的30只BALB／c裸鼠皮肤组织的细胞凋亡指数，应用免疫组化S-P法检测皮肤组织中caspase-8和cyt-c的表达。结果电镜结果显示高剂量组出现细胞调亡。40％、80％、100％TCE剂量组凋亡指数与对照组的差异有统计学意义（P〈0．01）；20％、40％、80％、100％TCE剂量组cyt c的表达水平分别为（2．60±0．54）、（3．42±0．56）、（5.81±1．30）、（6．00±0．70）明显高于对照组，差异均有统计学意义（P〈0．01），各组caspase-8表达差异无统计学意义（P〉0．05）。结论TCE引起的早期皮肤组织损伤与细胞凋亡有关，而细胞凋亡可能与线粒体损伤有关。Objective To investigate the expression of apoptosis and caspase-8,cyt c in the skin of the BALB/c mice exposed to trichloroethylene（TCE）. Methods 30 BALB/c mice were divided in random into the solvent control group, 10% TCE group, 20% TCE group,40% TCE group, 80% TCE group and 100% TCE group. Apoptotic cells were detected by TUNEL and EM. The expressions of caspase-8 and cyt c were detected with immunohistochemical method. Results EM showed that the apoptosis of cells was found in the high dosage groups. The immunohistochemical results showed that there were significant differences in the apoptosis rate and the aetivity of cyt c between the different dosage groups. There was the significant difference in the apoptosis rate between the 40%, 80%, 100% TCE groups the control group（P〈0.01 ）. There was the significant difference in the expression of cyt c between the 20%,40%, 80%, 100% TCE groups [（2.60±0.54）, （3.42±0.56）, （5.81±1.30）and （6.00±0.70）, respectively] and the control group（P〈0.01 ）. The expressions of caspase-8 had no significant differences （P〉0.05）. Conclusion Apoptosis plays an important role in trichloroethylene induced in＇itant injury in skin and the apoptosis may be related with the mitochondrial injury.

关 键 词：三氯乙烯 皮炎 变应性接触性 细胞色素C 

分 类 号：R686[医药卫生—骨科学]