矽肺患者诱导痰中NO2^-／NO3^-和硝化酪氨酸含量的变化  

Changes of NO2^-/NO3^- and nitration tyrosine concentrations in induced sputum of silicosis patients

作　　者：王坤[1] 王瑞[1] 刘正亮[1] 门金龙[1] 万恩广 孔令敏[3] 王金鹏[3] 沈超[3] 李扬[3] 

机构地区：[1]山东省职业卫生与职业病防治研究院,济南250062 [2]兖州矿业集团职业病防治所 [3]山东中医药大学第二附属医院

出　　处：《中华劳动卫生职业病杂志》2009年第1期30-33,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：山东省卫生厅计划课题（2005HW164）

摘　　要：目的分析矽肺患者和接尘工人诱导痰叶NO2^-/NO3^-、硝化酪氨酸的含量变化，探讨一氧化氮代谢产物作为矽肺早期炎性标志物的可行性，方法以80名矽尘接触工人为接尘组，84名矽肺患者为病例组，30名无矽尘接触史的后勤人员为对照组，测定诱导痰中一氧化氮代谢产物NO2^-／NO3^-、硝化酪氨酸的含量，并进行NO2^-／NO3^-、硝化酪氨酸水平与接尘工龄、肺功能各项指标的相关分析。结果接尘组诱导痰中NO2^-／NO3^-水平160.30（46．58）μmol／L]明显高于对照组136．90（22．28）μmol／L]，而病例组179．65（89．10）μmol／L]明显高于接尘组和对照组，差异均有统计学意义（P〈0．05）；接尘组和病例组诱导痰中NO2^-/NO3^-水平与接尘工龄呈正相关（r值分别为0．3733和0．3830，P〈0．05），与FVC％、FEV10%、PEF％、MEF25％、MEF50％均呈负相关（r值分别为0．1540、0．1723、0．1535、0．1485、0．1643，P〈0．05）。接尘组和病例组诱导痰中硝化酪氨酸水平分别为[3．51（0．46）nmol／L]和[3．48（0．49）nmol／L]，明显高于对照组，差异有统计学意义（P〈0．05）；诱导痰硝化酪氨酸水平与接尘工龄和肺通气功能指标皆无相关性（P〉0．05）。结论诱导痰中NO2^-／NO3^-水平与接尘工龄呈正相关，NO2^-／NO3^-作为矽肺早期炎性标忠物具有一定的可行性。Objective To analyze the change in nitration tyrosine,NO2^-/NO3^- level in induced sputum of silicosis patients aud dust exposure workers and to evaluate the approach and feasibility of nitric oxide（ NO ） metabolites as early detection indicators of silicosis. Methods Nitration tyrosine,NO2^-/NO3^- concentration in induced sputum of 80 dust exposure workers, 84 silicosis patients, 30 h）gistie personnel with no history of exposure to silica dust were determined and the relationship among Nitration tyrosine, NO2^-/NO3^- level and dust exposure years as well as pulmonary function tests were analyzed. Results NO2^-/NO3^- level among exposed group[60.30（ 46.58 ）μmol/l] was significantly higher than the control group[36.90（ 22.28 ）μmol/l], （ P〈0.05 ）, and the level of NO2^-/NO3^- among the cases [79.65 （89.10）μmol/l] was significantly higher than exposed group as well as the control group（P〈0.05 ）. Compared with control, the level of nitration tyrosine in exposed group[3.51 （0.46） nmol/l] and the cases[3.48（0.49 ）nmol/l] was significantly higher（P〈0.05 ）. NO2^-/NO3^- level and dust exposure years were positively correlated （ r=0.3733 and 0.3830 respectirely P〈0.05 ） ; NO2^-/NO3^- level and pulmonary function tests （ FVC%, FEV10%, PEF%, MEF25%, MEF50% ） were negatively correlated （ r=0.1540, 0.1723,0.1535,0.1485,0.1643 respectirely,P〈0.05 ）. There was no correlation between nitration tyrosine and dust exposure years （P〉0.05）, no correlation between nitration tyrosine and pulmonary function test （P〉0.05）. Conclusion The level of NO2^-/NO3^- level in induced sputum has a positive correlation with exposure to dust, suggesting that there will be a certain feasibility of the NO2^-/NO3^- as indicators of early detection of silicosis.

关 键 词：矽肺 一氧化氮 痰 硝酸盐类 酪氨酸 

分 类 号：R686[医药卫生—骨科学]