均匀设计优化聚谷氨酸与表阿霉素的交联条件  

Optimizing the conjugating condition of polyglutamic acid and epirubicin by uniform design

作　　者：徐凤华 蒋雪涛[1] 主皓 

机构地区：[1]第二军医大学药学院药剂教研室

出　　处：《中国医院药学杂志》1998年第5期214-215,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的：优化聚谷氨酸与表阿霉素的交联条件。方法：采用均匀实验设计，考察ａ．表阿霉素的投量（ｍｇ），ｂ．反应时间（ｈ），ｃ．反应体系的ｐＨ值对交联率的影响。结果：表阿霉素的交联率（Ｙ）在实验范围内与各因素之间符合方程：Ｙ＝７６．７０＋０．３３７８ａ－２．２８５×１０－２ｂ－８．１４１ｃ，γ＝０．９８８８。此方程在ｐＨ５．４以上适用，药物交联率最高可达到３９％。BJECTIVES: To optimize the conjugating condition of polyglutamic acid(PGA) and epirubicin(EPR).METHODS:Arranging three experimental factors according to uniform design:milligram of epirubicin involved,duration of the reaction(h),and the pH value of the reaction solution.RESULTS:The conjugating rate of epirubicin and the three experimental factors conform to the following equation:Y=76.70±0.3378a-2.286×10-2b-8.141c,r=0.988 8.The equition is adaptable when the pH value of the reaction solution is above 5.4,and the maximum conjugating rate can be up to 39 %.CONCLUSIONS:The influence of the experimental factors to the conjugating rate is determined quantitively.

关 键 词：表阿霉素 聚谷氨酸 交联 均匀设计 免疫偶合物 

分 类 号：R979.14[医药卫生—药品] R392－33[医药卫生—药学]